Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Size Exclusion Chromatography
Product nameRecombinant EN-TEV Protease protein
See all EN-TEV Protease proteins and peptides
The EZCut™ TEV Protease has an activity of ≥10 mU/mg
Purity>= 95 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
Actual molecular weight29 kDa including tags
Amino acids2038 to 2279
TagsHis tag C-Terminus
Our Abpromise guarantee covers the use of ab285976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Size Exclusion Chromatography
This product is manufactured by BioVision, an Abcam company and was previously called 7847 EZCut™ TEV Protease, Recombinant. 7847-1000 is the same size as the 1 mg size of ab285976.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped on Dry Ice. Store at -80°C.
Constituents: 3% Sodium chloride, 1.6% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.07% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.015% EDTA
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Cleavage of poly-his tagged control protein by TEV protease for 1h at 37°C
Lane 1: 10µg Poly his-control protein
Lane 2: 10µg Poly his-control protein + 10 mU TEV Protease
Lane 3: 10µg Poly his-control protein + 1 mU TEV Protease
Size exclusion chromatography analysis of ab285976 (after removal of DTT, EDTA and glycerol) using a Supersex 200 HR 10/30 column at 0.5ml/min in 50 mM Tris and 0.25M NaCl, pH 7.5.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab285976 has not yet been referenced specifically in any publications.