Recombinant EN-TEV Protease protein (ab285976)
Key features and details
- Expression system: Escherichia coli
- Purity: >= 95% SDS-PAGE
- Active: Yes
- Tags: His tag C-Terminus
- Suitable for: SDS-PAGE, Size Exclusion Chromatography
Description
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Product name
Recombinant EN-TEV Protease protein
See all EN-TEV Protease proteins and peptides -
Biological activity
The EZCut™ TEV Protease has an activity of ≥10 mU/mg
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Purity
>= 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Actual molecular weight
29 kDa including tags -
Amino acids
2038 to 2279 -
Tags
His tag C-Terminus
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Specifications
Our Abpromise guarantee covers the use of ab285976 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Size Exclusion Chromatography
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Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 7847 EZCut™ TEV Protease, Recombinant. 7847-1000 is the same size as the 1 mg size of ab285976.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Store at -80°C.
pH: 8.00
Constituents: 3% Sodium chloride, 1.6% Tris HCl, 20% Glycerol (glycerin, glycerine), 0.07% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.015% EDTAThis product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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SDS-PAGE - (ab285976)
Cleavage of poly-his tagged control protein by TEV protease for 1h at 37°C
Lane 1: 10µg Poly his-control protein
Lane 2: 10µg Poly his-control protein + 10 mU TEV Protease
Lane 3: 10µg Poly his-control protein + 1 mU TEV Protease
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Size Exclusion Chromatography - (ab285976)Size exclusion chromatography analysis of ab285976 (after removal of DTT, EDTA and glycerol) using a Supersex 200 HR 10/30 column at 0.5ml/min in 50 mM Tris and 0.25M NaCl, pH 7.5.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab285976 has not yet been referenced specifically in any publications.