Description

  • Product name

    Recombinant HIV tat protein
    See all HIV tat proteins and peptides
  • Purity

    > 98 % SDS-PAGE.

  • Expression system

    Escherichia coli
  • Accession

  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Amino acids

      1 to 101

Specifications

Our Abpromise guarantee covers the use of ab109949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot

    SDS-PAGE

  • Form

    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.

    Constituent: 0.1% Glycerol

  • Reconstitution
    The protein should be reconstituted in apirogenic sterile water or PBS buffer. The reconstituted solution has to be used immediately. (Avoid repeated freeze/thaw cycles).

General Info

  • Alternative names

    • p14
    • Tat
    • Tat protein
    • Transactivating regulatory protein
  • Relevance

    The transcriptional transactivator (Tat) is a key regulatory protein of HIV. It is expressed early after the virus integrates into the cell, and stimulates the elongation of RNA polymerase II. It binds onto a sequence known as the TAR, or transactivator response element, located at the end of the HIV genetic chain. There, the tat protein helps assemble new copies of HIV. The tat protein-TAR complex speeds up the rate of viral reproduction by about a thousand times. If it is not present, the transcription process frequently stops short, and few functional HIV particles are produced. Tat is an important potential target for antiretrovirals and vaccine development.

References

ab109949 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

We have as yet not used the HIV tat protein (ab109949) in ELISA so I cannot provide a protocol for how this application may be performed. This does not mean that it would not work, simply that we have not tested it ourselves and therefore would not be able to guarantee this application.

The protocol used to perform Western blotting with the protein is attached to this email. The quantity of ab109949 loaded in the 14% polyacrylamide gelwas 2.5µg-5µg.


If you have any further questions, please do not hesitate to contact us again.

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