For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

  1. Link

    recombinant-hiv1-protease-protein-ab84117.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Microbiology Organism Virus RNA Virus ssRNA positive strand virus HIV
Share by email

Recombinant HIV1 Protease protein (ab84117)

  • Datasheet
Submit a review Q&A (2)References (2)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Active: Yes
  • Tags: His tag C-Terminus
  • Suitable for: SDS-PAGE, Functional Studies

You may also be interested in

Protein
Product image
Recombinant human DLL4 protein (Fc Chimera Active) (ab108557)
Protein
Product image
Recombinant Staphylococcus alpha Hemolysin protein (Active) (ab233724)
Protein
Product image
Recombinant HIV1 gp41 protein (ab49070)

View more associated products

Description

  • Product name

    Recombinant HIV1 Protease protein
    See all HIV1 Protease proteins and peptides
  • Biological activity

    One unit of HIV Protease hydrolyzes 1 picomole of a peptide (SQNYPIVQ) per minute at pH 4.7 at 25oC. 20-200ng is sufficient for an in vitro protease assay. HIV Protease can be applied in in vitro assay development and screening of protease inhibitors.

  • Purity

    > 95 % SDS-PAGE.
    Purified by an affinity chromatography in combination with FPLC chromatography
  • Expression system

    Escherichia coli
  • Protein length

    Full length protein
  • Animal free

    No
  • Nature

    Recombinant
    • Sequence

      PQITLWQRPL VTIKIGGQLK EALLDTGADD TVLEEMSLPG RWKPKMIGGI GGFIKVRQYD QILIEICGHK AIGTVLVGPT PVNIIGRNLL TQIGCTLNF
    • Predicted molecular weight

      12 kDa
    • Amino acids

      1 to 99
    • Tags

      His tag C-Terminus

Associated products

  • Related Products

    • Anti-HIV protease antibody [1696] (ab8327)

Specifications

Our Abpromise guarantee covers the use of ab84117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Functional Studies

  • Form

    Liquid
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.9
    Constituents: 3.75% Potassium chloride, 0.0154% DTT, 0.316% Tris HCl, 0.00584% EDTA, 20% Glycerol (glycerin, glycerine)

    This product is an active protein and may elicit a biological response in vivo, handle with caution.

General Info

  • Alternative names

    • HIV-1 protease

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • Datasheet download

    Download

References (2)

Publishing research using ab84117? Please let us know so that we can cite the reference in this datasheet.

ab84117 has been referenced in 2 publications.

  • Imamura T  et al. Isolation of amaranthin synthetase from Chenopodium quinoa and construction of an amaranthin production system using suspension-cultured tobacco BY-2 cells. Plant Biotechnol J 17:969-981 (2019). PubMed: 30451369
  • Boso G  et al. The nature of the N-terminal amino acid residue of HIV-1 RNase H is critical for the stability of reverse transcriptase in viral particles. J Virol 89:1286-97 (2015). PubMed: 25392207

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-2 of 2 Abreviews or Q&A

Question

Phone call requesting information on if all the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are active or not.

Read More

Abcam community

Verified customer

Asked on Jun 29 2012

Answer

Thank you for contacting us yesterday and you interest in our products.

I have had a look into your query relating to whether the HIV protease proteins (ab113857, ab124313, ab124858 and ab84117) are all active. The title you have noticed where "Active" is included is down to an inconsistency in naming in our catalogue, it is not an indicator that ab113857 or ab124858 are not active. I am sorry for this confusion, I am going to try to have this amended.

Each one of the proteins have been shown to have enzyme activity through peptide cleavage assays. Details of these assays can be found on each of the individual datasheets of the products. This means that all of them should be suitable for your experiment if you are using a peptide sequence recognised by the protease. The experiment you are hoping to perform would be covered by the Abpromise guarantee. More information on this guarantee can be found from the following page:

https://www.abcam.com/abpromise

If you would like to try any of the proteins, I'd like to encourage you tosubmit anAbreview via the online product datasheet once you have used it in your assay. As a thank you you will be rewarded with Abpoints which can be used to redeem against Amazon vouchers or future purchases from us. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews

I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More

Abcam Scientific Support

Answered on Jun 29 2012

Question

One unit of ab84117 hydrolyzes 1 picomole of a peptide (SQNYPIVQ) per minute at pH 4.7 at 25oC. performed in which buffer?

Read More

Abcam community

Verified customer

Asked on Dec 23 2011

Answer

Thank you for your patience. I apologize for the delay in getting back to you. I just received the reply from the supplying lab today. "For our experiments measuring the enzymatic activity, we have used both i) 100 mM MES (pH6), 200 mM NaCl, 0.1 mM EDTA and 1 mM DTT ii) 100 mM NaOAc buffer (pH5.5), 1 M NaCl, 1mM EDTA, 1 mM DTT, 0.02% Tween 20 Based on the literature, also 100 mM NaoAc, pH 4.7, 1 M NaCl, 1 mM EDTA, 1mM DTT gives a high activity for HIV protease and can be used." I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Dec 23 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Get resources and offers direct to your inbox Sign up
A-Z by research area
  • Cancer
  • Cardiovascular
  • Cell biology
  • Developmental biology
  • Epigenetics & Nuclear signaling
  • Immunology
  • Metabolism
  • Microbiology
  • Neuroscience
  • Signal transduction
  • Stem cells
A-Z by product type
  • Primary antibodies
  • Secondary antibodies
  • Biochemicals
  • Isotype controls
  • Flow cytometry multi-color selector
  • Kits
  • Loading controls
  • Lysates
  • Peptides
  • Proteins
  • Slides
  • Tags and cell markers
  • Tools & Reagents
Help & support
  • Support
  • Make an Inquiry
  • Protocols & troubleshooting
  • Placing an order
  • RabMAb products
  • Biochemical product FAQs
  • Training
  • Browse by Target
Company
  • Corporate site
  • Investor relations
  • Company news
  • Careers
  • About us
  • Blog
Events
  • Tradeshows
  • Conferences
International websites
  • abcam.cn
  • abcam.co.jp

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
  • Modern slavery statement
© 1998-2022 Abcam plc. All rights reserved.