Overview

  • Product name
    Recombinant HPV16 L1 protein
  • Protein length
    Full length protein

Description

  • Nature
    Recombinant
  • Source
    Saccharomyces cerevisiae
  • Amino Acid Sequence
    • Accession
    • Molecular weight
      56 kDa
    • Amino acids
      1 to 505

Associated products

Specifications

Our Abpromise guarantee covers the use of ab119880 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    ELISA

    Western blot

    SDS-PAGE

    Electron Microscopy

  • Purity
    > 90 % SDS-PAGE.
    ab119880 is purified by ultracentifugation.
  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. After reconstitution store at -20ºC. Avoid freeze / thaw cycles.

    Constituent: 99% PBS

  • Reconstitution
    Reconstitute in 50 ul of water. Then dilute with PBS to the required concentration.

General Info

  • Alternative names
    • HPV-16
    • HPV-16 capsid
    • HPV16 capsid protein
    • HPV16 L1
    • HPV16 major capsid protein L1
    • Human papillomavirus type 16 L1
    • Human papillomavirus type 16 major capsid protein L1
    • L1
    • Major capsid L1 protein
    • Major capsid protein
    • Major capsid protein L1
    see all
  • Relevance
    L1 is a major capsid protein of type 16 human papilloma virus. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies.
  • Cellular localization
    Virion. Host Nucleus.

Images

  • SDS-PAGE analysis of ab119880.

    Lane 1. ab119880, 1 µg/lane
    Lane 2. MWt marker 11.0, 17.0, 26.0, 34.0, 43.0, 55.0, 72.0, 95.0, 130.0, 170.0 kDa.
  • ab119880 viewed by electron microscopy.

References

ab119880 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer



Below is a more detailed description of antigen quantification for these protein products:

The determination of the quantities of L1 proteins in each VLP preparation was based on the comparison with the known quantities of L1 (µL) and BSA proteins (µg) run in the same gel. Few quantities (1, 2, 3 µL per lane from the same VLP preparation) of HPV16 or HPV18 L1 VLPs were loaded on the SDS-PAGE gel next to BSA protein (0.1; 0,5; 1; 1,5, 2, 3 µg per lane). The quantities of L1 and BSA proteins loaded on the gel was determined by the densitometric scanning of the Coomassie brilliant blue stained protein bands fractionated by SDS-PAGE, using the ImageScanner III (GE Healthcare, Little Chalfont, UK ) device. Quantitative analysis was conducted with the ImageQuantTL software supplied with the instrument. At least three scans of each VLP preparation were obtained and average concentration was calculated.

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Answer

While preparing the CoC for these products, I realized that we have an important information for the customer: Indeed, some vials of the lot xx ab119880 might contain 100ug instead of 50ug.We had about a dozen vials, for which we are unfortunatelynot able to determine whether thevial contains 100ug or 50ug. The 4 vials you have ordered lately are part of these vials. I am very sorry. Can you please check with the customer whether he had any problems in this regards? Please let us know whether there have been any problems, or whether there might be any problems and I will do my best to solve this.

Thank you very much for your help. I look forward to hear back from you.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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