Recombinant Human Annexin-2/ANXA2 protein (ab93005)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Endotoxin level: < 0.250 Eu/ml
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, WB, ELISA
Description
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Product name
Recombinant Human Annexin-2/ANXA2 protein
See all Annexin-2/ANXA2 proteins and peptides -
Purity
> 95 % SDS-PAGE.
Purified by 6xHis tag / Immobilized-metal affinity chromatography (IMAC). -
Endotoxin level
< 0.250 Eu/ml -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Molecular weight information
41.3 kDa -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab93005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Mass Spectrometry
Western blot
ELISA
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Form
Liquid -
Additional notes
This product was previously labelled as Annexin A2
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.4
Constituent: 100% PBS
General Info
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Alternative names
- Annexin A2
- Annexin II
- Annexin II, heavy chain
see all -
Function
Calcium-regulated membrane-binding protein whose affinity for calcium is greatly enhanced by anionic phospholipids. It binds two calcium ions with high affinity. May be involved in heat-stress response. -
Sequence similarities
Belongs to the annexin family.
Contains 4 annexin repeats. -
Domain
A pair of annexin repeats may form one binding site for calcium and phospholipid. -
Post-translational
modificationsPhosphorylation of Tyr-24 enhances heat stress-induced translocation to the cell surface.
ISGylated. -
Cellular localization
Secreted > extracellular space > extracellular matrix > basement membrane. Melanosome. In the lamina beneath the plasma membrane. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Translocated from the cytoplasm to the cell surface through a Golgi-independent mechanism. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab93005 has been referenced in 4 publications.
- Liu T et al. Cancer-associated fibroblast-specific lncRNA LINC01614 enhances glutamine uptake in lung adenocarcinoma. J Hematol Oncol 15:141 (2022). PubMed: 36209111
- Klabklai P et al. Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence. Int J Mol Sci 23:N/A (2022). PubMed: 36293376
- Ye Q et al. The important roles and molecular mechanisms of annexin A2 autoantibody in children with nephrotic syndrome. Ann Transl Med 9:1452 (2021). PubMed: 34734004
- Vais H et al. ER-luminal [Ca2+] regulation of InsP3 receptor gating mediated by an ER-luminal peripheral Ca2+-binding protein. Elife 9:N/A (2020). PubMed: 32420875