Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE
Product nameRecombinant Human Band 3/AE 1 protein (Tagged)
Purity> 90 % SDS-PAGE.
Expression systemEscherichia coli
Protein lengthProtein fragment
SequenceMEELQDDYEDMMEENLEQEEYEDPDIPESQMEEPAAHDTEATATDYHTTS HPGTHKVYVELQELVMDEKNQELRWMEAARWVQLEENLGENGAWGRPHLS HLTFWSLLELRRVFTKGTVLLDLQETSLAGVANQLLDRFIFEDQIRPQDR EELLRALLLKHSHAGELEALGGVKPAVLTRSGDPSQPLLPQHSSLETQLF CEQGDGGTEGHSPSGILEKIPPDSEATLVLVGRADFLEQPVLGFVRLQEA AELEAVELPVPIRFLFVLLGPEAPHIDYTQLGRAAATLMSERVFRIDAYM AQSRGELLHSLEGFLDCSLVLPPTDAPSEQALLSLVPVQRELLRRRYQSS PAKPDSSFYKGLDLNGGPDDPLQQTGQLFGGLVRDIRRRYPYYLSDITDA FSP
Predicted molecular weight65 kDa including tags
Amino acids1 to 403
TagsHis tag N-Terminus
Additional sequence informationCytoplasmic domain. N-terminal 10xHis-SUMO-tag and C-terminal Myc-tag.
- Anti-Band 3/AE 1 antibody [EPR1426] (ab108414)
- Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)
- Anti-6X His tag® antibody [HIS.H8] (ab18184)
- Anti-Myc tag antibody [Myc.A7] (ab18185)
- Anti-6X His tag® antibody [4D11] (ab5000)
- Anti-Myc tag antibody (ab9106)
- Anti-6X His tag® antibody (ab9108)
- Anti-Myc tag antibody - ChIP Grade (ab9132)
Our Abpromise guarantee covers the use of ab235968 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Previously labelled as Band 3.
Concentration information loading...
Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
- AE 1
- Anion exchange protein 1
FunctionFunctions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine.
Tissue specificityDetected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871).
Involvement in diseaseOvalocytosis, Southeast Asian
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Sequence similaritiesBelongs to the anion exchanger (TC 2.A.31) family.
modificationsPhosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
Cellular localizationCell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney.
- Information by UniProt
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235968 has not yet been referenced specifically in any publications.