Recombinant Human Bax protein (Tagged) (ab85157)



  • Nature
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
    • Tags
      GST tag N-Terminus


Our Abpromise guarantee covers the use of ab85157 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Western blot


  • Form
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.

    pH: 7.50
    Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA

General Info

  • Alternative names
    • Apoptosis regulator BAX
    • BAX
    • Bax-protein
    • BAXA
    • Baxdelta2G9
    • Baxdelta2G9omega
    • Baxdelta2omega
    • Bcl-2-like protein 4
    • BCL2 associated X protein
    • BCL2 associated X protein omega
    • BCL2 associated X protein transcript variant delta2
    • Bcl2-L-4
    • BCL2L4
    • membrane isoform alpha
    see all
  • Function
    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • Tissue specificity
    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • Sequence similarities
    Belongs to the Bcl-2 family.
  • Domain
    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • Cellular localization
    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Information by UniProt


  • Lane 1 = Bax protein (Tagged) (ab85157) – 10 ng. Lane 2 = Extract of HeLa cells – 40 ug. Lane 3 = Extract of HepG2 cells – 40 ug. Lane 4 = Bax protein (Tagged) (ab85157) – 10 ng. Lane 5 = Extract of HeLa cells – 40 ug. Lane 6 = Extract of HepG2 cells – 40 ug. SDS PAGE performed under reducing conditions (100mM DTT – Sample heated at 50ºC). Primary : Lanes 1-3: Anti Bax antibody (ab77566) at 1 ug/mL. Lanes 4-6: Anti Bax antibody (ab32503) at 1:2000 dilution. Secondary : Lanes 1-3: Goat anti mouse IgG(H&L)-HRP at 1:10000. Lanes 4-6: Goat anti rabbit IgG(H&L)-HRP at 1:10000. Development: ECL with 2 min exposure. Blocking: in 5% Milk + PBS for 3 hours at RT. Primary antibody: in 5% Milk + PBS overnight at 4 C. Secondary antibody: in 5% Milk + PBS for 2 hour at RT. Predicted band size : Bax 21kDa and Bax (Tagged) 49 kDa. Observed band size : Bax 21kDa and Bax (Tagged) 49 kDa

  • SDS-PAGE showing ab85158 at approximately 49kDa.
  • Standard curve for Bax (Analyte: ab85157); dilution range 1pg/ml to 1µg/ml using Capture Antibody ab77566 at 0.2µg/ml and Detector Antibody ab32503 at 0.5µg/ml. Concentration of ab32503 may vary from lot to lot; please use this curve as guideline.


ab85157 has not yet been referenced specifically in any publications.

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