Overview

Description

  • Nature
    Recombinant
  • Source
    Escherichia coli
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      AA Sequence: MEKLLCFLVL TSLSHAFGQT DMSRKAFVFP KESDTSYVSL KAPLTKPLKA FTVCLHFYTE LSSTRGYSIF SYATKRQDNE ILIFWSKDIG YSFTVGGSEI LFEVPEVTVA PVHICTSWES ASGIVEFWVD GKPRVRKSLK KGYTVGAEAS IILGQEQDSF GGNFEGSQSL VGDIGNVNMW DFVLSPDEIN TIYLGGPFSP NVLNWRALKY EVQGEVFTKP QLWP
    • Molecular weight
      115 kDa
    • Amino acids
      1 to 224

Specifications

Our Abpromise guarantee covers the use of ab51784 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    Western blot

  • Purity
    > 95 % SDS-PAGE.
    >95% by SDS-PAGE, HPLC analysis and anion exchange FPLC.
  • Form
    Liquid
  • Additional notes

    Endotoxin level is ?<0.1 ng/μg.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA). Reconstitute for long term storage.

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.812% Sodium chloride, 0.242% Tris, 0.0222% Calcium chloride

General Info

  • Alternative names
    • Pentraxin 1, short
    • C reactive protein
    • C reactive protein pentraxin related
    • C-reactive protein(1-205)
    • CRP
    • CRP_HUMAN
    • MGC88244
    • Pentraxin 1
    • PTX 1
    • PTX1
    see all
  • Function
    Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
  • Tissue specificity
    Found in plasma.
  • Sequence similarities
    Belongs to the pentaxin family.
    Contains 1 pentaxin domain.
  • Cellular localization
    Secreted.
  • Information by UniProt

Images

  • Anti-C Reactive Protein antibody (ab78051) at 1 µg/ml + Recombinant Human C Reactive Protein (ab51784) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Performed under reducing conditions.

    Exposure time: 1 minute
  • 4-20% SDS-PAGE of Human CRP: 5 ug of Recombinant human CRP (C-reactive protein) loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant human CRP has a predicted MW of ~ 115 kDa corresponding to the pentameric structure of 23 kDa monomers.

References

ab51784 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
ELISA
CRP doesn’t recognized by monoclonal antibody anti-CRP.

Abcam user community

Verified customer

Submitted Oct 30 2018

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