Recombinant Human CD3 protein (Fc Chimera His Tag) (ab220590)

Overview

Description

  • Nature
    Recombinant
  • Source
    HEK 293 cells
  • Amino Acid Sequence
    • Accession
    • Species
      Human
    • Sequence
      DGNEEMGG ITQTPYKVSI SGTTVILTCP QYPGSEILWQ HNDKNIGGDE DDKNIGSDED HLSLKEFSEL EQSGYYVCYP RGSKPEDANF YLYLRARVCE NCMEMD
    • Molecular weight
      39 kDa including tags
    • Amino acids
      23 to 126
    • Tags
      His tag C-Terminus
    • Additional sequence information
      This protein carries a human IgG1 Fc tag at the C-terminus, followed by a polyhistidine tag.(NP_000724.1).

Specifications

Our Abpromise guarantee covers the use of ab220590 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

  • Endotoxin level
    < 1.000 Eu/µg
  • Purity
    >95% by SDS-PAGE .

  • Form
    Lyophilised
  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at -80°C. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 5% Trehalose, 95% PBS

    Lyophilized from 0.22 µm filtered solution.
    5-10% trehalose is commonly used for freeze drying, and after reconstitution, the trehalose is mostly about 3-5%

  • Reconstitution
    It is recommended to reconstitute the lyophilized protein in sterile deionized water to a final concentration of 1mg/ml. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Carrier protein (0.1% HSA or BSA) is strongly recommended for further dilution and long term storage.

General Info

  • Alternative names
    • 4930549J05Rik
    • A430104F18Rik
    • AW552088
    • Cd247
    • CD247 antigen
    • CD247 antigen, zeta subunit
    • CD247 molecule
    • CD3
    • CD3 antigen, delta subunit
    • CD3 delta
    • CD3 epsilon
    • CD3 eta
    • CD3 gamma
    • CD3 molecule delta polypeptide
    • CD3 molecule, epsilon polypeptide
    • CD3 molecule, gamma polypeptide
    • CD3 zeta
    • CD3-DELTA
    • CD3d
    • CD3D antigen delta polypeptide
    • CD3d antigen, delta polypeptide (TiT3 complex)
    • CD3d molecule delta
    • CD3d molecule delta CD3 TCR complex
    • CD3d molecule, delta (CD3-TCR complex)
    • CD3D_HUMAN
    • CD3E
    • CD3e antigen
    • CD3E antigen epsilon polypeptide
    • CD3e antigen, epsilon polypeptide (TiT3 complex)
    • CD3e molecule epsilon
    • CD3e molecule epsilon CD3 TCR complex
    • CD3e molecule, epsilon (CD3-TCR complex)
    • CD3epsilon
    • CD3G
    • CD3g antigen
    • CD3G antigen gamma polypeptide
    • CD3g antigen, gamma polypeptide (TiT3 complex)
    • CD3g molecule gamma
    • CD3g molecule gamma CD3 TCR complex
    • CD3g molecule, gamma (CD3-TCR complex)
    • CD3H
    • CD3Q
    • CD3Z
    • CD3zeta
    • Ctg3
    • FLJ17620
    • FLJ17664
    • FLJ18683
    • FLJ79544
    • FLJ94613
    • IMD19
    • Leu-4
    • MGC138597
    • OKT3, delta chain
    • OTTHUMP00000032544
    • T cell receptor
    • T cell receptor T3 delta chain
    • T cell receptor T3 gamma chain
    • T cell receptor T3 zeta chain
    • T cell receptor zeta chain
    • T cell surface glycoprotein CD3
    • T cell surface glycoprotein CD3 delta chain
    • T cell surface glycoprotein CD3 epsilon chain
    • T cell surface glycoprotein CD3 gamma chain
    • T cell surface glycoprotein CD3 zeta chain
    • T-cell antigen receptor complex, delta subunit of T3
    • T-cell antigen receptor complex, epsilon subunit of T3
    • T-cell antigen receptor complex, gamma subunit of T3
    • T-cell antigen receptor complex, zeta subunit of CD3
    • T-cell receptor T3 delta chain
    • T-cell receptor T3 gamma chain
    • T-cell surface antigen T3/Leu-4 epsilon chain
    • T-cell surface glycoprotein CD3 delta chain
    • T-cell surface glycoprotein CD3 gamma chain
    • T3
    • T3d
    • T3e
    • T3g
    • T3z
    • TCRE
    • TCRk
    • Tcrz
    • TCRzeta
    see all
  • Function
    The CD3 complex mediates signal transduction.
  • Involvement in disease
    Defects in CD3D are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T(-)/B(+)/NK(+) SCID) [MIM:608971]. A form of severe combined immunodeficiency (SCID), a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients present in infancy recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development.
  • Sequence similarities
    Contains 1 ITAM domain.
  • Cellular localization
    Membrane.
  • Information by UniProt

Images

  • SDS-PAGE analysis of reduced ab220590 stained overnight with Coomassie Blue.

    The protein migrates as 44-54 kDa under reducing conditions (SDS-PAGE) due to glycosylation.

References

ab220590 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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