Recombinant Human LATS2 protein (ab71357)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Suitable for: WB
Description
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Product name
Recombinant Human LATS2 protein
See all LATS2 proteins and peptides -
Purity
> 80 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Amino acids
480 to 1088
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Specifications
Our Abpromise guarantee covers the use of ab71357 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
General Info
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Alternative names
- FLJ13161
- Kinase phosphorylated during mitosis protein
- KPM
see all -
Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in centrosome duplication, maintenance of mitotic fidelity and genomic stability. Negatively regulates G1/S transition by down-regulating cyclin E/CDK2 kinase activity. Negative regulator of the androgen receptor. -
Tissue specificity
Expressed at high levels in heart and skeletal muscle and at lower levels in all other tissues examined. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Post-translational
modificationsAutophosphorylated and phosphorylated during M-phase and the G1/S-phase of the cell cycle. Phosphorylated and activated by STK3. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome. Cytoplasm. Cytoplasm > cytoskeleton > spindle pole. Co-localizes with STK6 at the centrosomes during interphase, early prophase and cytokinesis. Migrates to the spindle poles during mitosis, and to the midbody during cytokinesis. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab71357 has not yet been referenced specifically in any publications.