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Yes, these answers are of help.
I will test the protein using an electrophoretic mobility assay for
its affinity to its known DNA binding sequence. I must admit though
that I do not have a positive control because I do not have known
active pdx-1 currently available to me. If the test result is of use
to you despite the absence of a positive control, I am willing to
share the data obtained and participate in the test program.
Asked on May 14 2012
Yes, you can submit an Abreview for EMSA. Please select "Other" in the application field, and also please submit as much information as you are able to provide.
DISCOUNT CODE: xxx
Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab114175 in EMSA. This code will give you: 1 freePROTEINbefore the expiration date. To redeem this offer, please submit an Abreview for EMSAand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.
Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.
Abcam Scientific Support
Answered on May 14 2012