Recombinant Human RYK protein (ab139623)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Suitable for: WB, SDS-PAGE
Description
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Product name
Recombinant Human RYK protein
See all RYK proteins and peptides -
Purity
> 90 % SDS-PAGE.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
MKRIELDDSISASSSSQGLSQPSTQTTQYLRADTPNNATPITSSLGYPTL RIEKNDLRSVTLLEAKGKVKDIAISRERITLKDVLQEGTFGRIFHGILID EKDPNKEKQAFVKTVKDQASEIQVTMMLTESCKLRGLHHRNLLPITHVCI EEGEKPMVILPYMNWGNLKLFLRQCKLVEANNPQAISQQDLVHMAIQIAC GMSYLARREVIHKDLAARNCVIDDTLQVKITDNALSRDLFPMDYHCLGDN ENRPVRWMALESLVNNEFSSASDVWAFGVTLWELMTLGQTPYVDIDPFEM AAYLKDGYRIAQPINCPDELFAVMACCWALDPEERPKFQQLVQCLTEFHA ALGAYV -
Predicted molecular weight
66 kDa including tags -
Amino acids
255 to 610
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Specifications
Our Abpromise guarantee covers the use of ab139623 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.31% Glutathione, 0.002% PMSF, 0.004% DTT, 0.79% Tris HCl, 0.003% EDTA, 25% Glycerol (glycerin, glycerine), 0.29% Sodium chloride
General Info
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Alternative names
- D3S3195
- ERK 3
- Hydroxyaryl protein kinase
see all -
Function
May be a coreceptor along with FZD8 of Wnt proteins, such as WNT1, WNT3, WNT3A and WNT5A. Involved in neuron differentiation, axon guidance, corpus callosum establishment and neurite outgrowth. In response to WNT3 stimulation, receptor C-terminal cleavage occurs in its transmembrane region and allows the C-terminal intracellular product to translocate from the cytoplasm to the nucleus where it plays a crucial role in neuronal development. -
Tissue specificity
Observed in all the tissues examined. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 protein kinase domain.
Contains 1 WIF domain. -
Domain
The extracellular WIF domain is responsible for Wnt binding. -
Post-translational
modificationsProteolytically cleaved, in part by presenilin, in response to WNT3 stimulation. Cleavage occurs during neuronal differentiation. -
Cellular localization
Membrane. Nucleus. Cytoplasm. In cells that have undergone neuronal differentiation, the C-terminal cleaved part is translocated from the cytoplasm to the nucleus. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab139623 has not yet been referenced specifically in any publications.