Recombinant Mouse MUSK protein (Tagged) (ab226426)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Tags: His tag N-Terminus
- Suitable for: MS, SDS-PAGE
Description
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Product name
Recombinant Mouse MUSK protein (Tagged)
See all MUSK proteins and peptides -
Purity
> 90 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
EKLPKAPVITTPLETVDALVEEVATFMCAVESYPQPEISWTRNKILIKLF DTRYSIRENGQLLTILSVEDSDDGIYCCIANNGVGGAVESCGALQVKMKP KITRPPINVKIIEGLKAVLPCTTMGNPKPSVSWIKGDNALRENSRIAVLE SGSLRIHNVQKEDAGQYRCVAKNSLGTAYSKLVKLEVEVFARILRAPESH NVTFGSFVTLRCTAIGIPVPTISWIENGNAVSSGSIQESVKDRVIDSRLQ LFITKPGLYTCIATNKHGEKFSTAKAAATVSIAEWSKSQKDSQGYCAQYR GEVCDAVLAKDALVFFNTSYRDPEDAQELLIHTAWNELKAVSPLCRPAAE ALLCNHLFQECSPGVVPTPMPICREYCLAVKELFCAKEWQAMEGKAHRGL YRSGMHLLPVPECSKLPSMHRDPTACTRLPYLDYKKENITTFPSITS -
Predicted molecular weight
69 kDa including tags -
Amino acids
22 to 468 -
Tags
His tag N-Terminus -
Additional sequence information
6xHis-SUMO tag at the N-terminus and Myc tag at the C-terminus.
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab226426 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Mass Spectrometry
SDS-PAGE
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Mass spectrometry
LC-MS/MS -
Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
pH: 7.2
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
General Info
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Alternative names
- CMS9
- FADS
- MDK 4
see all -
Function
Receptor tyrosine kinase that is a key mediator of agrin's action and is involved in neuromuscular junction (NMJ) organization. -
Involvement in disease
Defects in MUSK is a cause of congenital myasthenic syndrome with acetylcholine receptor deficiency (CMS-ACHRD) [MIM:608931]. A post-synaptic congenital myasthenic syndrome. Mutations underlying AChR deficiency cause a 'loss of function' and show recessive inheritance. Note=MUSK mutations lead to decreased agrin-dependent AChR aggregation, a critical step in the formation of the neuromuscular junction. -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family.
Contains 1 FZ (frizzled) domain.
Contains 3 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
Post-translational
modificationsUbiquitinated by PDZRN3. Ubiquitination promotes endocytosis and lysosomal degradation. -
Cellular localization
Membrane. - Information by UniProt
Images
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(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab226426 with 5% enrichment gel and 15% separation gel.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab226426 could indicate that this peptide derived from E.coli-expressed mouse Musk.
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Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of ab226426 could indicate that this peptide derived from E.coli-expressed mouse Musk.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab226426 has not yet been referenced specifically in any publications.