Recombinant Mouse VEGFC protein (ab51947)
Key features and details
- Expression system: Escherichia coli
- Purity: > 90% SDS-PAGE
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant Mouse VEGFC protein
See all VEGFC proteins and peptides -
Purity
> 90 % SDS-PAGE.
>90% by SDS-PAGE analyses. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
HY NTEILKSIDN EWRKTQCMPR EVCIDVGKEF GAATNTFFKPPCVSVYRCGG CCNSEGLQCM NTSTGYLSKTLFEITVPLSQ GPKPVTISFANHTSCRCMSK LDVYRQVHSI IRR -
Amino acids
108 to 223
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Specifications
Our Abpromise guarantee covers the use of ab51947 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 4634 VEGF-C, murine recombinant. 4634-10 is the same size as the 10 µg size of ab51947.
Reconstituted VEGFC should be stored in working aliquots at -80°C.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituent: 0.1% Acetic acid
50 µg BSA per 1 µg VEGFC -
ReconstitutionWe recommend a quick spin followed by reconstitution in 0.1% acetic acid to a concentration of 0.1-1.0 mg/ml.
General Info
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Alternative names
- Flt 4L
- Flt4 ligand
- FLT4 ligand DHM
see all -
Function
Growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration and also has effects on the permeability of blood vessels. May function in angiogenesis of the venous and lymphatic vascular systems during embryogenesis, and also in the maintenance of differentiated lymphatic endothelium in adults. Binds and activates VEGFR-2 (KDR/FLK1) and VEGFR-3 (FLT4) receptors. -
Tissue specificity
Spleen, lymph node, thymus, appendix, bone marrow, heart, placenta, ovary, skeletal muscle, prostate, testis, colon and small intestine and fetal liver, lung and kidney, but not in peripheral blood lymphocyte. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Post-translational
modificationsUndergoes a complex proteolytic maturation which generates a variety of processed secreted forms with increased activity toward VEGFR-3, but only the fully processed form could activate VEGFR-2. VEGF-C first form an antiparallel homodimer linked by disulfide bonds. Before secretion, a cleavage occurs between Arg-227 and Ser-228 producing an heterotetramer. The next extracellular step of the processing removes the N-terminal propeptide. Finally the mature VEGF-C is composed mostly of two VEGF homology domains (VHDs) bound by non-covalent interactions. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (1)
ab51947 has been referenced in 1 publication.
- Nayar S et al. Bimodal Expansion of the Lymphatic Vessels Is Regulated by the Sequential Expression of IL-7 and Lymphotoxin a1ß2 in Newly Formed Tertiary Lymphoid Structures. J Immunol 197:1957-67 (2016). PubMed: 27474071