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Recombinant Mycobacterium tuberculosis ESAT6 protein (ab124574)

Reviews (1)Q&A (1)References (2)
SDS-PAGE - Recombinant Mycobacterium tuberculosis ESAT6 protein (ab124574)

    Key features and details

    • Expression system: Baculovirus
    • Purity: > 90% SDS-PAGE
    • Endotoxin level: < 1.000 Eu/µg
    • Tags: His tag C-Terminus
    • Suitable for: SDS-PAGE

    Description

    • Product name

      Recombinant Mycobacterium tuberculosis ESAT6 protein

    • Purity

      > 90 % SDS-PAGE.
      Affinity purified

    • Endotoxin level

      < 1.000 Eu/µg

    • Expression system

      Baculovirus

    • Accession

      P0A564

    • Protein length

      Full length protein

    • Animal free

      No

    • Nature

      Recombinant

      • Species

        Mycobacterium tuberculosis

      • Sequence

        ADPMTEQQWN FAGIEAAASA IQGNVTSIHS LLDEGKQSLT KLAAAWGGSG SEAYQGVQQK WDATATELNN ALQNLARTIS EAGQAMASTE GNVTGMFAHH HHHH

      • Predicted molecular weight

        11 kDa including tags

      • Amino acids

        1 to 95

      • Tags

        His tag C-Terminus

    Specifications

    Our Abpromise guarantee covers the use of ab124574 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      pH: 8.00
      Constituents: 0.32% Tris HCl, 10% Glycerol (glycerin, glycerine)

    General Info

    • Alternative names

      • 6 kDa early secretory antigenic target
      • 6 kDa early secretory antigenic target ESXA
      • ESAT 6
      • esaT6
      • ESXA
      see all

    • Relevance

      The ESAT6 antigen from Mycobacterium tuberculosis is a dominant target for cell mediated immunity in the early phase of tuberculosis (TB) in TB patients as well as in various animal models. ESAT6 is not found in M. bovis BCG. Its function is not known but it elicits high level of IFN-gamma from memory effector cells during the first phase of a protective immune response.

    • Cellular localization

      Secreted

    Images

    • SDS-PAGE - Recombinant Mycobacterium tuberculosis ESAT6 protein (ab124574)
      SDS-PAGE - Recombinant Mycobacterium tuberculosis ESAT6 protein (ab124574)

      SDS-PAGE analysis of ab124574 (3 µg) under reducing condition and visualized by coomassie blue stain.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (2)

    Publishing research using ab124574? Please let us know so that we can cite the reference in this datasheet.

    ab124574 has been referenced in 2 publications.

    • Sepulveda D  et al. Bioelectrochemical Detection of Mycobacterium tuberculosis ESAT-6 in an Antibody-Based Biomicrosystem. Sensors (Basel) 17:N/A (2017). PubMed: 28937645
    • Ouyang W  et al. Enabling electrical biomolecular detection in high ionic concentrations and enhancement of the detection limit thereof by coupling a nanofluidic crystal with reconfigurable ion concentration polarization. Lab Chip 17:3772-3784 (2017). PubMed: 28983543

    Customer reviews and Q&As

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    1-2 of 2 Abreviews or Q&A

    The efficiency of ESAT-6 as a stimulant of T-cells for IFN-g production.

     Excellent
    Abreviews
    abreview image
    Application
    Functional Studies
    The aim of this research was to test the ability of ESAT-6 to stimulate T-cell production of Interferon-gamma ,IFN-g.
    Blood from a consenting QuantiFERON positive participant was collected. Monocytes, CD4+ and CD8+ cells were isolated from the participant’s blood. The monocytes served as antigen presenting cells, therefore 100 000 monocytes were co-cultured with 100 000 CD4+ or CD8+ cells respectively. Thus, each well had a total of 200 000 cells and a final volume of 200ul.
    These co-cultures were then stimulated according to set conditions. Some cells were left unstimulated to serve as a negative control. Recombinant ESAT-6 ,ab124574 - Recombinant Mycobacterium tuberculosis ESAT6 protein from Abcam, was used to stimulate CD4+ and CD8+ cells at concentrations of 2.5ug/ml, 5ug/ml or 10ug/ml. Phytohemagglutinin -PHA- 10ug/ml, and BCG ,MOI 5:1, were used as positive control stimulants. The stimulated cultures were incubated for 72 hours at 37 C and 5% CO2.
    After 72 hours the supernatants were collected from each culture well. The IFN-γ concentration from each culture was quantified with Human IFN-g Quantikine ELISA. The results were expressed in a scatter plot depicting the concentration of IFN-g ,pg/ml, secreted by CD4+ and CD8+ cells in response to each stimulation condition ,Figure 1.
    The results show an increase in IFN-g production for all stimulation conditions compared to the unstimulated cultures. Upon comparing ESAT-6 at different concentrations, ESAT-6 at 10ug/ml induced the highest IFN-γ secretion for both CD4+ and CD8+ cells.
    It can be concluded that ESAT-6 can be used as a stimulant in a stimulation assay to activate T cells to produce IFN-g.
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Stacey Engel

    Verified customer

    Submitted Aug 07 2020

    Question



    I would like to purchase ab124574 and expect it has biological activity. May i ask how the protein was expressed and purified? Thank you for your help!

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    Answer

    Thank you for contacting us.

    ab124574 is a recombinant full length mycobacterium tuberculosis ESAT6 expressed by Baculovirus and purified by using conventional chromatography.


    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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