Key features and details
- Purity: >= 99% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies
Product nameRecombinant Proteinase K
See all Proteinase K proteins and peptides
≥ 30 U/mg
Purity>= 99 % SDS-PAGE.
Protein lengthFull length protein
Actual molecular weight29 kDa
Our Abpromise guarantee covers the use of ab286002 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product is manufactured by BioVision, an Abcam company and was previously called 9210 Proteinase K, Recombinant, Molecular Grade. 9210-10g is the same size as the 10 g size of ab286002.
A serine protease that displays the ability to digest native proteins, thereby inactivating enzymes such as DNase and RNase without recourse to a denaturation process. It retains its activity in presence of SDS and urea. It is inactivated by diisopropyl fluorophosphates (DFP) and phenyl methane sulfonyl fluoride (PMSF).
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Preparation and Storage
Stability and Storage
Shipped at 4°C. Store at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
ReconstitutionCentrifuge the vial prior to opening. Soluble in 50 mM Tris-HCl (pH 7.5), 3 mM CaCl2, 50% Glycerol yielding a clear colorless solution
- Endopeptidase K
- Tritirachium alkaline proteinase
RelevanceProteinase K is an endolytic protease that cleaves peptide bonds preferentially next to the carboxyl group of N-substituted hydrophobic aliphatic and aromatic amino acids. Proteinase K is classified as a serine protease and exhibits broad substrate specificity. It can hydrolyze keratin; hence the name. Proteinase K is used for the digestion of proteins on tissue sections as a pre-treatment to aid in the preparation of mRNA and its detection by in situ hybridisation methods using oligonucleotide probes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab286002 has not yet been referenced specifically in any publications.