Recombinant human ABL1 protein (ab69810)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 70% Affinity purified
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant human ABL1 protein
See all ABL1 proteins and peptides -
Biological activity
Specific activity: 871 nmol/min/mg
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Purity
> 70 % Affinity purified.
Purified by affinity chromatography -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
EALQRPVASD FEPQGLSEAA RWNSKENLLA GPSENDPNLF VALYDFVASG DNTLSITKGE KLRVLGYNHN GEWCEAQTKN GQGWVPSNYI TPVNSLEKHS WYHGPVSRNA AEYLLSSGIN GSFLVRESES SPGQRSISLR YEGRVYHYRI NTASDGKLYV SSESRFNTLA ELVHHHSTVA DGLITTLHYP APKRNKPTVY GVSPNYDKWE MERTDITMKH KLGEGHFGEV YEGVWKKYSL TVAVKTLKED TMEVEEFLKE AAVMKEIKHP NLVQLLGVCT REPPFYIITE FMTYGNLLDY LRECNRQEVN AVVLLYMATQ ISSAMEYLEK KNFIHRDLAA RNCLVGENHL VKVADFGLSR LMTGDTYTAH AGAKFPIKWT APESLAYNKF SIKSDVWAFG VLLWEIATYG MSPYPGIDLS QVYELLEKDY RMERPEGCPE KVYELMRACW QWNPSDRPSF AEIHQAFETM FQESSISDEV EKELGKQGVR GAVSTLLQAP ELPTKTRTSR RAAEHRDTTD VPEMPHSKGQ GESDPLDHEP AVSPLLPRKE RGPPEGGLNE DERLLPKDKK TNLFSALIKK KKKTAPTPPK RSSSFREMDG QPERRGAGEE EGRDISNGAL AFTPLDTADP AKSPKPSNGA GVPNGALRES GGSGFRSPHL WKKSSTLTSS RLATGEEEGG GSSSKRFLRS CSASCVPHGA KDTEWRSVTL PRDLQSTGRQ FDSSTFGGHK SEKPALPRKR AGENRSDQVT RGTVTPPPRL VKKNEEAADE VFKDIMESSP GSSPPNLTPK PLRRQVTVAP ASGLPHKEEA GKGSALGTPA AAEPVTPTSK AGSGAPGGTS KGPAEESRVR RHKHSSESPG RDKGKLSRLK PAPPPPPAAS AGKAGGKPSQ SPSQEAAGEA VLGAKTKATS LVDAVNSDAA KPSQPGEGLK KPVLPATPKP QSAKPSGTPI SPAPVPSTLP SASSALAGDQ PSSTAFIPLI STRVSLRKTR QPPERIASGA ITKGVVLDST EALCLAISRN SEQMASHSAV LEAGKNLYTF CVSYVDSIQQ MRNKFAFREA INKLENNLRE LQICPATAGS GPAATQDFSK LLSSVKEISD IVQR -
Predicted molecular weight
135 kDa -
Amino acids
27 to 1130 -
Tags
His tag N-Terminus
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Associated products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab69810 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Liquid -
Additional notes
ab204848 (ABL1 peptide) can be utilized as a substrate for assessing Kinase activity
Previously labelled as c Abl.
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- Abelson murine leukemia viral oncogene homolog 1
- Abelson tyrosine protein kinase 1
- ABL
see all -
Function
Protein kinase that regulates key processes linked to cell growth and survival. Regulates cytoskeleton remodeling during cell differentiation, cell division and cell adhesion. Localizes to dynamic actin structures, and phosphorylates CRK and CRKL, DOK1, and other proteins controlling cytoskeleton dynamics. Regulates DNA repair potentially by activating the proapoptotic pathway when the DNA damage is too severe to be repaired. Phosphorylates PSMA7 that leads to an inhibition of proteasomal activity and cell cycle transition blocks. -
Tissue specificity
Widely expressed. -
Involvement in disease
Note=A chromosomal aberration involving ABL1 is a cause of chronic myeloid leukemia. Translocation t(9;22)(q34;q11) with BCR. The translocation produces a BCR-ABL found also in acute myeloid leukemia (AML) and acute lymphoblastic leukemia (ALL). -
Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. ABL subfamily.
Contains 1 protein kinase domain.
Contains 1 SH2 domain.
Contains 1 SH3 domain. -
Post-translational
modificationsPhosphorylated by PRKDC (By similarity). DNA damage-induced activation of c-Abl requires the function of ATM and Ser-446 phosphorylation (By similarity). Phosphorylation on Thr-735 is required for binding 14-3-3 proteins for cytoplasmic translocation.
Isoform IB is myristoylated on Gly-2. -
Cellular localization
Cytoplasm > cytoskeleton. Nucleus. Sequestered into the cytoplasm through interaction with 14-3-3 proteins and Nucleus membrane. The myristoylated c-ABL protein is reported to be nuclear. - Information by UniProt
Images
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The specific activity of ABL1 (ab69810) was determined to be 765.8 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab69810
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SDS PAGE analysis of ab69810
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Kinase activity assay of ab69810. The specific activity of c-Abl was determined to be 871 nmol/min/mg.
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SDS-PAGE of ab69810. Molecular weight 135kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab69810 has been referenced in 1 publication.
- Campbell H et al. SHP-2 is activated in response to force on E-cadherin and dephosphorylates vinculin Y822. J Cell Sci 131:N/A (2018). PubMed: 30478196