Recombinant human BACE1 protein (Active) (ab168075)
Key features and details
- Expression system: Insect cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE, Functional Studies
Description
-
Product name
Recombinant human BACE1 protein (Active)
See all BACE1 proteins and peptides -
Biological activity
Determined using a ß-Secretase Activity Assay Kit. Specific units > 120x103 units/mg. One unit is defined as the amount of enzyme that produces 1 pmol/min EDANS by hydrolyzing the substrate BVT9964 (NH2-Arg-Glu(EDANS)-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DABCYL)-Arg-COOH) at 37°C in 100 mM acetate buffer pH 4.0.
-
Purity
> 90 % SDS-PAGE. -
Expression system
Insect cells -
Accession
-
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
-
Species
Human -
Predicted molecular weight
47 kDa -
Amino acids
46 to 460 -
Additional sequence information
Mature protease domain.
-
Specifications
Our Abpromise guarantee covers the use of ab168075 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
Applications
SDS-PAGE
Functional Studies
-
Form
Liquid -
Additional notes
This product is manufactured by BioVision, an Abcam company and was previously called 7609 Active Recombinant Human beta-Secretase 1 - BACE-1. 7609-5 is the same size as the 5 µg size of ab168075.
Produced using non-baculovirus insect cells.
-
Concentration information loading...
Preparation and Storage
-
Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Constituents: 50% Glycerol (glycerin, glycerine), PBS
This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
-
Alternative names
- APP beta secretase
- Asp 2
- ASP2
see all -
Function
Responsible for the proteolytic processing of the amyloid precursor protein (APP). Cleaves at the N-terminus of the A-beta peptide sequence, between residues 671 and 672 of APP, leads to the generation and extracellular release of beta-cleaved soluble APP, and a corresponding cell-associated C-terminal fragment which is later released by gamma-secretase. -
Tissue specificity
Expressed at high levels in the brain and pancreas. In the brain, expression is highest in the substantia nigra, locus coruleus and medulla oblongata. -
Sequence similarities
Belongs to the peptidase A1 family. -
Domain
The transmembrane domain is necessary for its activity. It determines its late Golgi localization and access to its substrate, APP. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. Golgi apparatus > trans-Golgi network. Endoplasmic reticulum. Endosome. Cell surface. Predominantly localized to the later Golgi/trans-Golgi network (TGN) and minimally detectable in the early Golgi compartments. A small portion is also found in the endoplasmic reticulum, endosomes and on the cell surface. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
-
SDS download
-
Datasheet download
References (0)
ab168075 has not yet been referenced specifically in any publications.