Recombinant human MST4 protein (ab60341)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
Description
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Product name
Recombinant human MST4 protein -
Purity
> 90 % SDS-PAGE.
Purity: >90% as determined by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Human
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Associated products
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Related Products
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Substrate reagent
Specifications
Our Abpromise guarantee covers the use of ab60341 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
Western blot
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Form
Liquid -
Additional notes
ab43614 (Human Myelin Basic Protein full length protein) can be utilized as a substrate for assessing Kinase activity
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Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.0038% EGTA, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 2610018G03Rik
- EC 2.7.11.1
- Mammalian Ste20 like protein kinase 4
see all -
Function
Mediator of cell growth. Modulates apoptosis. -
Sequence similarities
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.
Contains 1 protein kinase domain. -
Cellular localization
Cytoplasm. Golgi apparatus membrane. Localizes to the Golgi apparatus. - Information by UniProt
Images
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The specific activity of MST4 (ab60341) was determined to be 23 nmol/min/mg as per activity assay protocol
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SDS PAGE analysis of ab60341
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Sample Kinase Activity Plot.
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Anti-MST4 antibody [EP1864Y] (ab52491) at 1/20000 dilution +
Recombinant human MST4 protein (ab60341) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Exposure time: 20 seconds
Ab52491 recognizes the full length tagged recombinant MST4 protein (ab60341) which has an expected molecular weight of 72 kDa.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab60341 has not yet been referenced specifically in any publications.