Recombinant human SIRT6 protein (ab104030)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 90% SDS-PAGE
- Active: Yes
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human SIRT6 protein
See all SIRT6 proteins and peptides -
Biological activity
The Specific activity of ab104030 was determined to be 120 RLU/min/ng.
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Purity
> 90 % SDS-PAGE.
Purity was determined to be >90% by densitometry. Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Human -
Sequence
IFDPPEELER KVWELARLVW QSSSVVFHTG AGISTASGIP DFRGPHGVWT MEERGLAPKF DTTFESARPT QTHMALVQLE RVGLLRFLVS QNVDGLHVRS GFPRDKLAEL HGNMFVEECA KCKTQYVRDT VVGTMGLKAT GRLCTVAKAR GLRACRGELR DTILDWEDSL PDRDLALADE ASRNADLSIT LGTSLQIRPS GNLPLATKRR GGRLVIVNLQ PTKHDRHADL RIHGYVDEVM TRLMKHLGLE IPAWDGPRVL ERALPPLPRP PTPKLEPKEE SPTRINGSIP AGPKQEPCAQ HNGSEPASPK RERPTSPAPH RPPKRVKAKA VPS -
Predicted molecular weight
39 kDa including tags -
Amino acids
23 to 355 -
Tags
His tag N-Terminus
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Associated products
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Related Products
Specifications
Our Abpromise guarantee covers the use of ab104030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.00
Preservative: 1.02% Imidazole
Constituents: 0.00174% PMSF, 0.82% Sodium phosphate, 0.00385% DTT, 25% Glycerol (glycerin, glycerine), 1.74% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- 2810449N18Rik
- AI043036
- Mono ADP ribosyltransferase sirtuin 6
see all -
Function
NAD-dependent protein deacetylase. Has deacetylase activity towards histone H3K9Ac and H3K56Ac. Modulates acetylation of histone H3 in telomeric chromatin during the S-phase of the cell cycle. Deacetylates histone H3K9Ac at NF-kappa-B target promoters and may down-regulate the expression of a subset of NF-kappa-B target genes. Acts as a corepressor of the transcription factor HIF1A to control the expression of multiple glycolytic genes to regulate glucose homeostasis. Required for genomic stability. Regulates the production of TNF protein. Has a role in the regulation of life span (By similarity). Deacetylation of nucleosomes interferes with RELA binding to target DNA. May be required for the association of WRN with telomeres during S-phase and for normal telomere maintenance. Required for genomic stability. Required for normal IGF1 serum levels and normal glucose homeostasis. Modulates cellular senescence and apoptosis. On DNA damage, promotes DNA end resection via deacetylation of RBBP8. Has very weak deacetylase activity and can bind NAD(+) in the absence of acetylated substrate. -
Sequence similarities
Belongs to the sirtuin family. Class IV subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Cellular localization
Nucleus, nucleoplasm. Predominantly nuclear. Associated with telomeric heterochromatin regions. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab104030 has not yet been referenced specifically in any publications.