Recombinant human SRGAP3 + BRAF protein (ab205991)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Tags: GST tag N-Terminus
- Suitable for: SDS-PAGE, Functional Studies
Description
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Product name
Recombinant human SRGAP3 + BRAF protein -
Biological activity
The specific activity of ab205991 was determined to be 250 nmol/min/mg.
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Purity
> 80 % Densitometry.
Affinity purified. -
Expression system
Baculovirus infected Sf9 cells -
Accession
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Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Amino Acid Sequence 1
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Species
Human -
Sequence
MSSQTKFKKDKEIIAEYEAQIKEIRTQLVEQFKCLEQQSESRLQLLQDLQ EFFRRKAEIELEYSRSLEKLAERFSSKIRSSREHQFKKDQYLLSPVNCWY LVLHQTRRESRDHATLNDIFMNNVIVRLSQISEDVIRLFKKSKEIGLQMH EELLKVTNELYTVMKTYHMYHAESISAESKLKEAEKQEEKQFNKSGDLSM NLLRHEDRPQRRSSVKKIEKMKEKRQAKYSENKLKCTKARNDYLLNLAAT NAAISKYYIHDVSDLIDCCDLGFHASLARTFRTYLSAEYNLETSRHEGLD VIENAVDNLDSRSDKHTVMDMCNQVFCPPLKFEFQPHMGDEVCQVSAQQP VQTELLMRYHQLQSRLATLKIENEEVRKTLDATMQTLQDMLTVEDFDVSD AFQHSRSTESVKSAASETYMSKINIAKRRANQQETEMFYFTKFKEYVNGS NLITKLQAKHDLLKQTLGEGERAECGTTRPPCLPPKPQKMRRPRPLSVYS HKLFNGSMEAFIKDLIRDQGFRGDGGSTTGLSATPPASLPGSLTNV KALQKSPGPQRERKSSSSSEDRNRMKTLGRRDSSDDWEIPDGQITVGQRI GSGSFGTVYKGKWHGDVAVKMLNVTAPTPQQLQAFKNEVGVLRKTRHVNI LLFMGYSTKPQLAIVTQWCEGSSLYHHLHIIETKFEMIKLIDIARQTAQG MDYLHAKSIIHRDLKSNNIFLHEDLTVKIGDFGLATVKSRWSGSHQFEQL SGSILWMAPEVIRMQDKNPYSFQSDVYAFGIVLYELMTGQLPYSNINNRD QIIFMVGRGYLSPDLSKVRSNCPKAMKRLMAECLKKKRDERPLFPQILAS IELLARSLPKIHRSASEPSLNRAGFQTEDFSLYACASPKTPIQAGGYGAF PVH
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Predicted molecular weight
128 kDa including tags -
Amino acids
1 to 513 -
Tags
GST tag N-Terminus
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Amino Acid Sequence 2
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Species
Human -
Amino acids
381 to 766
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Specifications
Our Abpromise guarantee covers the use of ab205991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
Functional Studies
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped on Dry Ice. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
pH: 7.50
Constituents: 0.79% Tris HCl, 0.87% Sodium chloride, 0.31% Glutathione, 0.003% EDTA, 0.004% DTT, 0.002% PMSF, 25% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
Images
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The specific activity of SRGAP3 + BRAF (ab205991) was determined to be 118 nmol/min/mg as per activity assay protocol and was equivalent to 252 nmol/min/mg as per radiometric assay
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SDS PAGE analysis of ab205991
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SDS PAGE analysis of ab205991
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SDS-PAGE analysis of ab205991.
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Sample Kinase Activity Plot. The specific activity of ab205991 was determined to be 250 nmol/min/mg.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab205991 has not yet been referenced specifically in any publications.