Recombinant mouse GDNF protein (ab56286)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE
Description
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Product name
Recombinant mouse GDNF protein
See all GDNF proteins and peptides -
Biological activity
Biological Activity : The ED50 was determined by the proliferation of rat C6 cells is = 0.2 ng/ml, corresponding to a specific activity of = 5 x 106 units/mg.
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Purity
> 95 % SDS-PAGE.
Endotoxin level is less than 0.1 ng per µg (1EU/µg). -
Expression system
Escherichia coli -
Protein length
Protein fragment -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MSPDKQAAAL PRRERNRQAA AASPENSRGK GRRGQRGKNR GCVLTAIHLN VTDLGLGYET KEELIFRYCS GSCESAETMY DKILKNLSRS RRLTSDKVGQ ACCRPVAFDD DLSFLDDNLV YHILRKHSAK RCGCI -
Amino acids
79 to 211
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Specifications
Our Abpromise guarantee covers the use of ab56286 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Functional Studies
SDS-PAGE
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Form
Lyophilized -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstituted GDNF is stable for at least 3 months when stored in working aliquots with a carrier protein at -20oC.
General Info
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Alternative names
- Astrocyte derived trophic factor
- Astrocyte derived trophic factor 1
- Astrocyte-derived trophic factor
see all -
Function
Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake. -
Tissue specificity
In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen. -
Involvement in disease
Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. -
Sequence similarities
Belongs to the TGF-beta family. GDNF subfamily. -
Cellular localization
Secreted. - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (2)
ab56286 has been referenced in 2 publications.
- Klimovich P et al. Three-Dimensional Model of Dorsal Root Ganglion Explant as a Method of Studying Neurotrophic Factors in Regenerative Medicine. Biomedicines 8:N/A (2020). PubMed: 32138155
- Rota C et al. Human amniotic fluid stem cell preconditioning improves their regenerative potential. Stem Cells Dev : (2011). PubMed: 22066606