Recombinant mouse PDGF AA protein (ab78590)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: SDS-PAGE
Description
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Product name
Recombinant mouse PDGF AA protein
See all PDGF AA proteins and peptides -
Biological activity
Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 8-10 ng/ml.
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Purity
> 95 % SDS-PAGE.
Purity Greater than 97% as determined by reducing and non-reducing SDS-PAGE, and by analytical HPLC. This product was sterile filtered. -
Expression system
Escherichia coli -
Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Mouse -
Sequence
MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT
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Specifications
Our Abpromise guarantee covers the use of ab78590 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
SDS-PAGE
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Form
Lyophilized -
Additional notes
It is recommended to add a carrier protein (0.1% HSA or BSA) for long term storage. -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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ReconstitutionReconstitute the lyophilized product with sterile water at a concentration of 0.1 -0.5 mg's/ml, which can be further diluted into other aqueous solutions.
General Info
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Alternative names
- PDGF A
- PDGF A chain
- PDGF subunit A
see all -
Function
Platelet-derived growth factor is a potent mitogen for cells of mesenchymal origin. Binding of this growth factor to its receptor elicits a variety of cellular responses. It is released by platelets upon wounding and plays an important role in stimulating adjacent cells to grow and thereby heals the wound. -
Sequence similarities
Belongs to the PDGF/VEGF growth factor family. -
Domain
The long form contains a basic insert which acts as a cell retention signal. -
Cellular localization
Secreted. - Information by UniProt
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (0)
ab78590 has not yet been referenced specifically in any publications.