Recombinant rat DNA Polymerase beta protein (ab123225)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% SDS-PAGE
- Active: Yes
- Suitable for: WB, Functional Studies, SDS-PAGE
Description
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Product name
Recombinant rat DNA Polymerase beta protein
See all DNA Polymerase beta proteins and peptides -
Biological activity
Enzyme activity: 90 unit/µl (1unit of the enzyme activity incorporates 1 nanomole of dNTP into acid-insoluble fraction at 37°C in 60 min.)
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Purity
> 95 % SDS-PAGE. -
Expression system
Escherichia coli -
Accession
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Protein length
Full length protein -
Animal free
No -
Nature
Recombinant -
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Species
Rat -
Predicted molecular weight
38 kDa -
Amino acids
2 to 335
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Specifications
Our Abpromise guarantee covers the use of ab123225 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Applications
Western blot
Functional Studies
SDS-PAGE
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Form
Liquid -
Concentration information loading...
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
pH: 6
Constituents: 2.28% Potassium chloride, 0.02% DTT, 0.79% Tris HCl, 0.003% EDTA, 20% Glycerol (glycerin, glycerine)This product is an active protein and may elicit a biological response in vivo, handle with caution.
General Info
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Alternative names
- DNA directed DNA polymerase beta
- DNA pol beta
- DNA polymerase beta
see all -
Function
Repair polymerase. Conducts "gap-filling" DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. Has a 5'-deoxyribose-5-phosphate lyase (dRP lyase) activity. -
Sequence similarities
Belongs to the DNA polymerase type-X family. -
Domain
Residues 239-252 form a flexible loop which appears to affect the polymerase fidelity. -
Post-translational
modificationsMethylation by PRMT6 stimulates the polymerase activity by enhancing DNA binding and processivity. -
Cellular localization
Nucleus, Cytoplasm - Information by UniProt
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab123225 has been referenced in 1 publication.
- Date T et al. Expression of active rat DNA polymerase beta in Escherichia coli. Biochemistry 27:2983-90 (1988). PubMed: 3042024