Anti-Renin Receptor antibody (ab64957)
Key features and details
- Rabbit polyclonal to Renin Receptor
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Renin Receptor antibody
See all Renin Receptor primary antibodies -
Description
Rabbit polyclonal to Renin Receptor -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human Renin Receptor (internal sequence).
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Positive control
- Human brain tissue COLO205 and HeLa cell extracts HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64957 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa).
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IHC-P | (1) |
1/50 - 1/100.
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ICC/IF |
1/500 - 1/1000.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 39 kDa (predicted molecular weight: 39 kDa). |
IHC-P
1/50 - 1/100. |
ICC/IF
1/500 - 1/1000. |
Target
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Function
Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS). -
Tissue specificity
Expressed in brain, heart, placenta, liver, kidney and pancreas. Barely detectable in lung and skeletal muscles. In the kidney cortex it is restricted to the mesangium of glomeruli. In the coronary and kidney artery it is expressed in the subendothelium, associated to smooth muscles where it colocalizes with REN. Expressed in vascular structures and by syncytiotrophoblast cells in the mature fetal placenta. -
Involvement in disease
Defects in ATP6AP2 are a cause of mental retardation X-linked with epilepsy (MRXE) [MIM:300423]. MRXE is a syndromic mental retardation. Patients manifest mild to moderate mental retardation associated with epilepsy, delays in motor milestones and speech acquisition in infancy. -
Post-translational
modificationsPhosphorylated. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 10159 Human
- Entrez Gene: 70495 Mouse
- Entrez Gene: 302526 Rat
- Omim: 300556 Human
- SwissProt: O75787 Human
- SwissProt: Q9CYN9 Mouse
- SwissProt: Q6AXS4 Rat
- Unigene: 495960 Human
see all -
Alternative names
- APT6M8 9 antibody
- APT6M8-9 antibody
- ATP6AP2 antibody
see all
Images
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All lanes : Anti-Renin Receptor antibody (ab64957) at 1/500 dilution
Lane 1 : COLO205 cell extract
Lane 2 : HeLa cell extract
Lane 3 : HeLa cell extract with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 39 kDa
Observed band size: 39 kDa -
Immunohistochemical analysis of paraffin-embedded human brain tissue using ab64957 at 1/50 dilution. Right hand panel was also treated with immunising peptide.
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Immunofluorescence analysis of HeLa cells, using ab64957 at 1/500 dilution. Right hand panel was also treated with immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab64957 has been referenced in 5 publications.
- Alzayadneh EM & Chappell MC Nuclear expression of renin-angiotensin system components in NRK-52E renal epithelial cells. J Renin Angiotensin Aldosterone Syst N/A:N/A (2014). ICC/IF . PubMed: 24961503
- White AJ et al. Expression of classical components of the renin-angiotensin system in the human eye. J Renin Angiotensin Aldosterone Syst N/A:N/A (2014). IHC ; Human . PubMed: 25287897
- Shi P et al. Direct pro-inflammatory effects of prorenin on microglia. PLoS One 9:e92937 (2014). IHC ; Mouse . PubMed: 25302502
- Pringle KG et al. The expression and localization of the human placental prorenin/renin-angiotensin system throughout pregnancy: roles in trophoblast invasion and angiogenesis? Placenta 32:956-62 (2011). PubMed: 22018415
- Marques FZ et al. Molecular characterization of renin-angiotensin system components in human intrauterine tissues and fetal membranes from vaginal delivery and cesarean section. Placenta 32:214-21 (2011). PubMed: 21215447