Anti-Retinal S antigen antibody [S128] (ab190315)
Key features and details
- Mouse monoclonal [S128] to Retinal S antigen
- Suitable for: WB, IHC-Fr
- Reacts with: Cow, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Retinal S antigen antibody [S128]
See all Retinal S antigen primary antibodies -
Description
Mouse monoclonal [S128] to Retinal S antigen -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Frmore details -
Species reactivity
Reacts with: Cow, Pig
Predicted to work with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Cow Retinal S antigen. (With the first 20 amino acids of the C-terminus truncated).
Database link: P08168 -
Positive control
- WB: Bovine retinal extract. ICC/IF: Pig retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
S128 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab190315 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa).
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IHC-Fr |
Use at an assay dependent concentration.
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Notes |
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WB
1/5000. Detects a band of approximately 48 kDa (predicted molecular weight: 45 kDa). |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Arrestin is one of the major proteins of the ros (retinal rod outer segments); it binds to photoactivated-phosphorylated rhodopsin, thereby apparently preventing the transducin-mediated activation of phosphodiesterase. -
Tissue specificity
Retina and pineal gland. -
Involvement in disease
Defects in SAG are a cause of congenital stationary night blindness Oguchi type 1 (CSNBO1) [MIM:258100]; also known as Oguchi disease. Congenital stationary night blindness is a non-progressive retinal disorder characterized by impaired night vision. CSNBO is an autosomal recessive form associated with fundus discoloration and abnormally slow dark adaptation. -
Sequence similarities
Belongs to the arrestin family. - Information by UniProt
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Database links
- Entrez Gene: 280922 Cow
- Entrez Gene: 6295 Human
- Entrez Gene: 20215 Mouse
- Entrez Gene: 397151 Pig
- Entrez Gene: 25539 Rat
- Omim: 181031 Human
- SwissProt: P08168 Cow
- SwissProt: P10523 Human
see all -
Alternative names
- 48 kDa protein antibody
- Arrestin 1 antibody
- Arrestin antibody
see all
Images
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Anti-Retinal S antigen antibody [S128] (ab190315) at 1/5000 dilution + Bovine retinal extract
Predicted band size: 45 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted? -
Confocal analysis of pig retina labeling Retinal S antigen with ab190315 at 1/1000 dilution (green). The red stain is Fox2, an RNA binding nuclear protein related to Fox3/NeuN, which stains nuclei of horizontal neurons and some other neurons in the INL and IPL. Nuclear DNA was revealed with DAPI (blue).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab190315 has not yet been referenced specifically in any publications.