Recombinant Anti-Ribophorin I antibody [EPR17044(B)] (ab197888)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17044(B)] to Ribophorin I
- Suitable for: WB
- Reacts with: Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Ribophorin I antibody [EPR17044(B)]
See all Ribophorin I primary antibodies -
Description
Rabbit monoclonal [EPR17044(B)] to Ribophorin I -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, BxPC-3, C6, and PC-12 whole cell lysates. Rat kidney and spleen lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17044(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab197888 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000. Predicted molecular weight: 69 kDa.
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Notes |
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WB
1/2000. Predicted molecular weight: 69 kDa. |
Target
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Function
Essential subunit of N-oligosaccharyl transferase enzyme which catalyzes the transfer of a high mannose oligosaccharide from a lipid-linked oligosaccharide donor to an asparagine residue within an Asn-X-Ser/Thr consensus motif in nascent polypeptide chains. -
Tissue specificity
Expressed in all tissues tested. -
Sequence similarities
Belongs to the OST1 family. -
Cellular localization
Endoplasmic reticulum membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 6184 Human
- Entrez Gene: 25596 Rat
- Omim: 180470 Human
- SwissProt: P04843 Human
- SwissProt: P07153 Rat
- Unigene: 518244 Human
- Unigene: 603636 Human
- Unigene: 4224 Rat
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Alternative names
- D6Wsu137e antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit antibody
- Dolichyl diphosphooligosaccharide protein glycosyltransferase 67 kDa subunit precursor antibody
see all
Images
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All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/20000 dilution
Lane 1 : BxPC-3 (human Pancreas adenocarcinoma ) whole cell lysate
Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Exposure time: 1 minuteBlocking and diluting buffer: 5% NFDM/TBST
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All lanes : Anti-Ribophorin I antibody [EPR17044(B)] (ab197888) at 1/2000 dilution
Lane 1 : Rat kidney lysate
Lane 2 : Rat spleen lysate
Lane 3 : C6 (Rat glial tumor cells) whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 69 kDa
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (0)
ab197888 has not yet been referenced specifically in any publications.