Key features and details
- Ca2+ release modulator
- CAS Number: 15662-33-6
- Soluble in ethanol to 10 mM
- Form / State: Solid
- Source: Ryania speciosa
Product nameRyanodine, Ca2+ release modulator
DescriptionCa2+ release modulator
Alkaloid that binds with high affinity to ryanodine receptors to modulate intracellular Ca2+ release. Has complex actions and may stimulate or inhibit Ca2+ release, depending on the concentration used.
Chemical name1H-Pyrrole-2-carboxylic acid (3S,4R,4aR,6S,7S,8R,8aS,8bR,9S,9aS)-dodecahydro-4,6,7,8a,8b,9a-hexahydroxy-3,6a,9-trimethyl-7-(1-methylethyl)-6,9-methanobenzo[1,2] pentaleno[1,6-bc]furan-8-yl ester
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in ethanol to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Refer to SDS for further information.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
2D chemical structure image of ab120083, Ryanodine, Ca2+ release modulator
Ryanodine inhibits the elevation of intracellular Ca2+ by PI(3,5)P2 in primary cardiac myocytes. Top figure shows fura-2 ratiometric changes in intracellular Ca2+ in an isolated ventricular adult cardiac myocyte after treatment with PI(3,5)P2, ultimately resulting in contraction. Bottom figure shows that ryanodine inhibited the release of SR Ca2+ to both caffeine and PI(3,5)P2.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab120083 has been referenced in 36 publications.
- King CM et al. Local Resting Ca2+ Controls the Scale of Astroglial Ca2+ Signals. Cell Rep 30:3466-3477.e4 (2020). PubMed: 32160550
- Sahu G et al. Junctophilin Proteins Tether a Cav1-RyR2-KCa3.1 Tripartite Complex to Regulate Neuronal Excitability. Cell Rep 28:2427-2442.e6 (2019). PubMed: 31461656
- Chen T et al. ROS-Mediated Mitochondrial Dysfunction and ER Stress Contribute to Compression-Induced Neuronal Injury. Neuroscience 416:268-280 (2019). PubMed: 31425734
- Luo R et al. Impaired calcium homeostasis via advanced glycation end products promotes apoptosis through endoplasmic reticulum stress in human nucleus pulposus cells and exacerbates intervertebral disc degeneration in rats. FEBS J 286:4356-4373 (2019). PubMed: 31230413
- Harari E et al. Direct Targeting of the mTOR (Mammalian Target of Rapamycin) Kinase Improves Endothelial Permeability in Drug-Eluting Stents-Brief Report. Arterioscler Thromb Vasc Biol 38:2217-2224 (2018). PubMed: 30026269