Product nameAnti-Ryanodine Receptor antibody [C3-33]
See all Ryanodine Receptor primary antibodies
DescriptionMouse monoclonal [C3-33] to Ryanodine Receptor
Tested applicationsSuitable for: ICC/IF, IHC-Fr, ICC, Flow Cyt, IHC-P, IP, WBmore details
Species reactivityReacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Fish, Amphibian
Predicted to work with: Horse, Pig
Full length protein corresponding to Dog Ryanodine Receptor. Canine cardiac ryanodine receptor.
Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab2868 as a replacement.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
PurityProtein G purified
Purification notesfrom ascites
Primary antibody notesThe Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
Our Abpromise guarantee covers the use of ab2827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-Fr||Use a concentration of 1 µg/ml.|
|Flow Cyt||Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IHC-P||Use a concentration of 1 µg/ml. Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.|
|IP||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 µg/ml. This antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.|
FunctionCalcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
Tissue specificitySkeletal muscle and brain (cerebellum and hippocampus).
Involvement in diseaseMalignant hyperthermia 1
Central core disease of muscle
Multiminicore disease with external ophthalmoplegia
Myopathy, congenital, with fiber-type disproportion
Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
Sequence similaritiesBelongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
Contains 3 B30.2/SPRY domains.
Contains 5 MIR domains.
DomainThe calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
modificationsChannel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
Cellular localizationSarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
- Information by UniProt
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ab2827 at 1/100 staining rabbit atrium tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG antibody was used as the secondary.
ab2827 staining Ryanodine Receptor in dog skeletal muscle section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone. The primary antibody was diluted 1/100 and incubated with sample for 12 hours at 4°C. A FITC-conjugated goat polyclonal to mouse IgG1, diluted 1/200 was used as secondary.
This product has been referenced in:
- Koivumäki JT et al. Structural Immaturity of Human iPSC-Derived Cardiomyocytes:In SilicoInvestigation of Effects on Function and Disease Modeling. Front Physiol 9:80 (2018). Read more (PubMed: 29467678) »
- Schneider C et al. The Anti-Cancer Multikinase Inhibitor Sorafenib Impairs Cardiac Contractility by Reducing Phospholamban Phosphorylation and Sarcoplasmic Calcium Transients. Sci Rep 8:5295 (2018). Read more (PubMed: 29593308) »