Overview

  • Product name
    Anti-Ryanodine Receptor antibody [C3-33]
    See all Ryanodine Receptor primary antibodies
  • Description
    Mouse monoclonal [C3-33] to Ryanodine Receptor
  • Host species
    Mouse
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, ICC, Flow Cyt, IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Chicken, Guinea pig, Dog, Human, Fish, Amphibian
    Predicted to work with: Horse, Pig
  • Immunogen

    Full length protein corresponding to Dog Ryanodine Receptor. Canine cardiac ryanodine receptor.

  • General notes

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibody ab2868 as a replacement.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    from ascites
  • Primary antibody notes
    The Ryanodine Receptor (RyR) is the channel responsible for the release of calcium from the sarcoplasmic reticulum (SR) in muscle cells and also plays a role in calcium regulation in non-muscle cells. The RyR exists as a homotetramer and is predicted to have a short cytoplasmic C-terminus and 4-10 transmembrane domains; the remainder of the protein, termed the "foot" region is located in the cytoplasm between the T-tubule and the SR. The mammalian RyR is the product of three different genes: RyR 1, which is expressed predominantly in skeletal muscle and areas of the brain, RyR 2, which is expressed predominantly in the heart muscle but also found in the stomach, endothelial cells and diffuse areas of the brain, and RyR-3 which is found in smooth muscle and the brain (striatum, thalamus and hippocampus).
  • Clonality
    Monoclonal
  • Clone number
    C3-33
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab2827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use a concentration of 1 µg/ml.
ICC 1/50.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

IHC-P Use a concentration of 1 µg/ml. Staining of RyR in rat cardiac tissue results in intense staining of the myofiber, which is consistent with sarcoplasmic reticulum localization.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. This antibody detects a 565 kDa protein representing the ryanodine receptor. In non-mammalian vertebrates, a doublet is seen at 565 kDa representing the alpha and beta isoforms of the receptor.

Target

  • Function
    Calcium channel that mediates the release of Ca(2+) from the sarcoplasmic reticulum into the cytoplasm and thereby plays a key role in triggering muscle contraction following depolarization of T-tubules. Repeated very high-level exercise increases the open probability of the channel and leads to Ca(2+) leaking into the cytoplasm. Can also mediate the release of Ca(2+) from intracellular stores in neurons, and may thereby promote prolonged Ca(2+) signaling in the brain. Required for normal embryonic development of muscle fibers and skeletal muscle. Required for normal heart morphogenesis, skin development and ossification during embryogenesis.
  • Tissue specificity
    Skeletal muscle and brain (cerebellum and hippocampus).
  • Involvement in disease
    Malignant hyperthermia 1
    Central core disease of muscle
    Multiminicore disease with external ophthalmoplegia
    Myopathy, congenital, with fiber-type disproportion
    Defects in RYR1 may be a cause of Samaritan myopathy, a congenital myopathy with benign course. Patients display severe hypotonia and respiratory distress at birth. Unlike other congenital myopathies, the health status constantly improves and patients are minimally affected at adulthood.
  • Sequence similarities
    Belongs to the ryanodine receptor (TC 1.A.3.1) family. RYR1 subfamily.
    Contains 3 B30.2/SPRY domains.
    Contains 5 MIR domains.
  • Domain
    The calcium release channel activity resides in the C-terminal region while the remaining part of the protein constitutes the 'foot' structure spanning the junctional gap between the sarcoplasmic reticulum (SR) and the T-tubule.
  • Post-translational
    modifications
    Channel activity is modulated by phosphorylation. Phosphorylation at Ser-2843 may increase channel activity. Repeated very high-level exercise increases phosphorylation at Ser-2843.
    Activated by reversible S-nitrosylation. Repeated very high-level exercise increases S-nitrosylation.
  • Cellular localization
    Sarcoplasmic reticulum membrane. Membrane. The number of predicted transmembrane domains varies between orthologs, but both N-terminus and C-terminus seem to be cytoplasmic.
  • Information by UniProt
  • Database links
  • Alternative names
    • Arrhythmogenic right ventricular dysplasia 2 antibody
    • ARVC2 antibody
    • ARVD2 antibody
    • Brain ryanodine receptor calcium release channel antibody
    • Brain type ryanodine receptor antibody
    • Cardiac muscle ryanodine receptor calcium release channel antibody
    • Cardiac muscle type ryanodine receptor antibody
    • CCO antibody
    • Central core disease of muscle antibody
    • HBRR antibody
    • hRYR 2 antibody
    • hRYR2 antibody
    • MHS antibody
    • MHS1 antibody
    • Ryanodine receptor 1 (skeletal) antibody
    • Ryanodine receptor 1 antibody
    • Ryanodine receptor 2 (cardiac) antibody
    • Ryanodine receptor 2 antibody
    • Ryanodine receptor 3 antibody
    • Ryanodine receptor type1 antibody
    • RYDR antibody
    • RYR 1 antibody
    • RYR 2 antibody
    • RYR 3 antibody
    • RYR antibody
    • RYR-1 antibody
    • RyR1 antibody
    • RYR1_HUMAN antibody
    • RYR2 antibody
    • RYR3 antibody
    • Sarcoplasmic reticulum calcium release channel antibody
    • Skeletal muscle calcium release channel antibody
    • Skeletal muscle ryanodine receptor antibody
    • Skeletal muscle type ryanodine receptor antibody
    • Skeletal muscle-type ryanodine receptor antibody
    • SKRR antibody
    • Type 1 like ryanodine receptor antibody
    • Type 1 ryanodine receptor antibody
    • VTSIP antibody
    see all

Images

  • ab2827 at 1/100 staining rabbit atrium tissue sections by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum prior to incubation with the antibody for 12 hours. An Alexa-Fluor ® 594 conjugated goat anti-mouse IgG antibody was used as the secondary.

    See Abreview

  • ab2827 staining Ryanodine Receptor in dog skeletal muscle section by Immunohistochemistry (Frozen sections). Tissue underwent fixation in acetone. The primary antibody was diluted 1/100 and incubated with sample for 12 hours at 4°C. A FITC-conjugated goat polyclonal to mouse IgG1, diluted 1/200 was used as secondary.

    See Abreview

References

This product has been referenced in:
  • Koivumäki JT  et al. Structural Immaturity of Human iPSC-Derived Cardiomyocytes:In SilicoInvestigation of Effects on Function and Disease Modeling. Front Physiol 9:80 (2018). Read more (PubMed: 29467678) »
  • Schneider C  et al. The Anti-Cancer Multikinase Inhibitor Sorafenib Impairs Cardiac Contractility by Reducing Phospholamban Phosphorylation and Sarcoplasmic Calcium Transients. Sci Rep 8:5295 (2018). Read more (PubMed: 29593308) »
See all 24 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Sheep Tissue lysate - whole (cardiac)
Specification
cardiac
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 15 2013

Question
Answer

Thank you for your reply.

Your credit note ID is **.

I am sorry that this antibody did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department so that we may gather the correct information needed for the refund. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used.

Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

The credit note ID is for your reference only and does not automatically guarantee the credit.

I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

Read More

Answer

Thank you for contacting Abcam.

I am sorry about the issues you were having with ab2877 in western blot. As the antibody is covered under our Abpromise for six months and is guaranteed to work in western blot on rat samples and I do not think that protocol tips would help resolve the issue, I would be happy to either send a replacement antibody or to process a refund.

Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody that would be very helpful.

I look forward to your reply.

Read More

Question
Answer

Thank you for contacting Abcam.
I am sorry about the issues you have been having with ab2827 in IF on cultured rat cells. The antibody is covered under our Abpromise for six months and is guaranteed to work in ICC/IF on rat samples.
Based on the information and the images that you provided, I do not think that protocol tips would help resolve the issue that you are having and so I would like to offer to either replace or refund the cost of the antibody. A potential replacement antibody would be:
https://www.abcam.com/Ryanodine-Receptor-antibody-34C-ab2868.html
Please let me know how you would you like to continue and if you could also provide either the Abcam order number or the PO# used to purchase the antibody that would be very helpful.
I look forward to your reply.

Read More
Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Skeletal muscles)
Specification
Skeletal muscles
Fixative
Acetone
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hESC-derived cardiomyocyte)
Specification
hESC-derived cardiomyocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 18 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (skeletal muscle)
Specification
skeletal muscle
Fixative
Acetone
Permeabilization
No

Abcam user community

Verified customer

Submitted May 20 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Tissue sections (atrium)
Specification
atrium
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%

Abcam user community

Verified customer

Submitted Apr 04 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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