Anti-S100 antibody [B32.1] (ab7852)
Key features and details
- Mouse monoclonal [B32.1] to S100
- Suitable for: IHC-FoFr
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-S100 antibody [B32.1]
See all S100 primary antibodies -
Description
Mouse monoclonal [B32.1] to S100 -
Host species
Mouse -
Specificity
This antibody reacts with alpha and beta subunits of human S-100 protein (21-24 kD). -
Tested applications
Suitable for: IHC-FoFrmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) corresponding to Cow S100.
Database link: P02639 -
Positive control
- Melanoma tissues containing nerves, such as intestine and pancreas. Brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: 0.01% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
B32.1 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7852 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-FoFr |
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4.
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Notes |
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IHC-FoFr
1/500 - 1/1000. 1/500 - 1/1000. Fix with 4% w/v paraformaldehyde and 15% saturated picric acid in 0.1 M. phosphate buffer pH 7.4. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. -
Tissue specificity
Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain. -
Sequence similarities
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 6271 Human
- Omim: 176940 Human
- SwissProt: P23297 Human
- Unigene: 515715 Human
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Alternative names
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
see all
Images
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ab7852 at a dilution of 1/1000, staining Astrocytes (purple; Alexa 488 secondary at 1/2000) on 30
µ m coronal brain section tissue in free floating IHC (see protocol link for detailed description). [A] Image taken with 20x objective and [B] Image taken with 40x objective. Images coloured in Photoshop.NB: No labeling observed following omission of primary antibody.
Sections were viewed using an Axioplan 2 Imaging microscope (Imaging Associates) fitted with 10x, 20x and 40x Plan-Neofluorobjectives (Zeiss, Germany) and images were taken using a AxioCam Hrm digital camera (Zeiss, Germany) and AxioVision software (Imaging Associates).
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab7852 has been referenced in 17 publications.
- Galiakberova AA et al. IPSC-Derived Human Neurons with GCaMP6s Expression Allow In Vitro Study of Neurophysiological Responses to Neurochemicals. Neurochem Res 47:952-966 (2022). PubMed: 34855047
- Gomez-Sintes R et al. Targeting retinoic acid receptor alpha-corepressor interaction activates chaperone-mediated autophagy and protects against retinal degeneration. Nat Commun 13:4220 (2022). PubMed: 35864098
- Arotcarena ML et al. Pilot Study Assessing the Impact of Intrathecal Administration of Variants AAV-PHP.B and AAV-PHP.eB on Brain Transduction in Adult Rhesus Macaques. Front Bioeng Biotechnol 9:762209 (2021). PubMed: 34869273
- Alhindi A et al. Terminal Schwann cells at the human neuromuscular junction. Brain Commun 3:fcab081 (2021). PubMed: 33977269
- Wu P et al. Comprehensive strategy of conduit guidance combined with VEGF producing Schwann cells accelerates peripheral nerve repair. Bioact Mater 6:3515-3527 (2021). PubMed: 33842738