Anti-S100 beta antibody - Astrocyte Marker (ab227914)
Key features and details
- Rabbit polyclonal to S100 beta - Astrocyte Marker
- Suitable for: IHC-P, WB, IHC-Fr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-S100 beta antibody - Astrocyte Marker
See all S100 beta primary antibodies -
Description
Rabbit polyclonal to S100 beta - Astrocyte Marker -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, IHC-Frmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Cow, Rhesus monkey -
Immunogen
Recombinant full length protein corresponding to Human S100 beta aa 1 to the C-terminus.
Database link: P04271 -
Positive control
- WB: S100 beta transfected HEK-293T whole cell extracts; A-375 whole cell extracts; Mouse and rat brain tissue extracts. IHC-P: Mouse hind brain tissue. IHC-Fr: Adult mouse retina tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 11 kDa.
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IHC-Fr |
1/100 - 1/1000.
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Notes |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 11 kDa. |
IHC-Fr
1/100 - 1/1000. |
Target
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Function
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. Binds to and initiates the activation of STK38 by releasing autoinhibitory intramolecular interactions within the kinase. Interaction with AGER after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling. -
Tissue specificity
Although predominant among the water-soluble brain proteins, S100 is also found in a variety of other tissues. -
Sequence similarities
Belongs to the S-101 family.
Contains 2 EF-hand domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 525716 Cow
- Entrez Gene: 6285 Human
- Entrez Gene: 20203 Mouse
- Entrez Gene: 100009495 Rabbit
- Entrez Gene: 25742 Rat
- Entrez Gene: 708117 Rhesus monkey
- Omim: 176990 Human
- SwissProt: P02638 Cow
see all -
Alternative names
- NEF antibody
- Protein S100 B antibody
- Protein S100-B antibody
see all
Images
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Frozen sections of adult mouse retina tissue stained for S100 beta using ab227914 at 1/250 dilution in immunohistochemical analysis.
Counter stain: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody at 1/250 dilution (red).
Nuclear counter stain: Fluoroshield with DAPI (blue). -
All lanes : Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution
Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with V5-human S100 beta, whole cell extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Paraffin-embedded mouse hind brain tissue stained for S100 beta using ab227914 at 1/500 dilution in immunohistochemical analysis.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/500 dilution + A-375 (human malignant melanoma cell line) whole cell extract at 30 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Mouse brain tissue extract at 50 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.
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Anti-S100 beta antibody - Astrocyte Marker (ab227914) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Predicted band size: 11 kDa15% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab227914 has been referenced in 2 publications.
- Ifuku M et al. Activation of Toll-like receptor 5 in microglia modulates their function and triggers neuronal injury. Acta Neuropathol Commun 8:159 (2020). PubMed: 32912327
- Todd L et al. Reactive microglia and IL1ß/IL-1R1-signaling mediate neuroprotection in excitotoxin-damaged mouse retina. J Neuroinflammation 16:118 (2019). PubMed: 31170999