• Product name

  • Description

    Goat polyclonal to SA1
  • Host species

  • Tested applications

    Suitable for: WB, ICC, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human, Xenopus laevis
    Predicted to work with: Mouse, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human SA1 aa 25-75. The exact sequence is proprietary.



Our Abpromise guarantee covers the use of ab4455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 144 kDa (predicted molecular weight: 144 kDa).
ICC 1/500 - 1/2000.
ICC/IF 1/500.
IP Use at an assay dependent concentration. PubMed: 20124417


  • Function

    Component of cohesin complex, a complex required for the cohesion of sister chromatids after DNA replication. The cohesin complex apparently forms a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. The cohesin complex may also play a role in spindle pole assembly during mitosis.
  • Sequence similarities

    Belongs to the SCC3 family.
    Contains 1 SCD (stromalin conservative) domain.
  • Post-translational

    Phosphorylated by PLK. The large dissociation of cohesin from chromosome arms during prophase is partly due to its phosphorylation.
  • Cellular localization

    Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of cohesin is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cohesin subunit SA 1 antibody
    • Cohesin subunit SA-1 antibody
    • Cohesin Subunit SA1 antibody
    • Nuclear protein stromal antigen 1 antibody
    • SA 1 antibody
    • SCC 3 homolog 1 antibody
    • Scc3 antibody
    • SCC3 homolog 1 antibody
    • STAG 1 antibody
    • stag1 antibody
    • STAG1_HUMAN antibody
    • Stromal antigen 1 antibody
    see all


  • Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4455 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.

    Nuclear Extract from HeLa cells (~5 x 105 cells).

    ab4455 at 0.5 µg/ml.

    Detection: ECL with 15 second exposure.
  • Immunocytochemistry analysis of HeLa cells (extracted for 5 minutes at 4°C in 0.5% Triton in CSK buffer) labelling SA1 with ab4455 at 1µg/ml.
  • Goat polyclonal to SA1 antibody (ab4455) at a 1/500 dilution detecting Stromal Antigen 1 in assynchronous HeLa cells. Cells were fixed with 4% paraformaldehyde and counterstained with DAPI. Secondary antibody:Donkey anti-goat IgG conjugated to Cy3. For further information please refer to abreview.

    See Abreview


This product has been referenced in:

  • Hopkins J  et al. Meiosis-specific cohesin component, Stag3 is essential for maintaining centromere chromatid cohesion, and required for DNA repair and synapsis between homologous chromosomes. PLoS Genet 10:e1004413 (2014). WB ; Mouse . Read more (PubMed: 24992337) »
  • Xiao T  et al. Specific Sites in the C Terminus of CTCF Interact with the SA2 Subunit of the Cohesin Complex and Are Required for Cohesin-Dependent Insulation Activity. Mol Cell Biol 31:2174-83 (2011). WB ; Human . Read more (PubMed: 21444719) »
See all 3 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence
Human Cell (HeLa)

Dr. Kirk Mcmanus

Verified customer

Submitted Apr 16 2007


Thank you very much for your patience. I'm afraid that I am unable to get this information from the originator of ab4455 and ab4457. I apologize for the inconvenience and if I can be of further assistance, please do let me know.

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Thank you for your email. All the information that we have regarding these antibodies is located on the online datasheets. I unfortunately can't tell you which one works "best." Are you detecting SA1 or SA2? The differences between the two SA1 antibodies and the two SA2 antibodies are the immunogens. The antibodies are fairly new and we have not had feedback from customers yet. If you have any more questions, please contact us again.

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