Anti-SAE1 (phospho S185) antibody (ab106096)
Key features and details
- Rabbit polyclonal to SAE1 (phospho S185)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAE1 (phospho S185) antibody
See all SAE1 primary antibodies -
Description
Rabbit polyclonal to SAE1 (phospho S185) -
Host species
Rabbit -
Specificity
ab106096 shows minimal reactivity by ELISA against the non-phosphorylated form of the immunising peptide -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Dog, Chimpanzee -
Immunogen
Synthetic peptide corresponding to Human SAE1 (phospho S185).
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Positive control
- HeLa cells treated with phosphatase inhibitors.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.42% Potassium phosphate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106096 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/3000. Predicted molecular weight: 38 kDa.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 38 kDa. |
Target
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Function
The heterodimer acts as a E1 ligase for SUMO1, SUMO2, SUMO3, and probably SUMO4. It mediates ATP-dependent activation of SUMO proteins followed by formation of a thioester bond between a SUMO protein and a conserved active site cysteine residue on UBA2/SAE2. -
Tissue specificity
Expression level increases during S phase and drops in G2 phase (at protein level). -
Pathway
Protein modification; protein sumoylation. -
Sequence similarities
Belongs to the ubiquitin-activating E1 family. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 505512 Cow
- Entrez Gene: 476425 Dog
- Entrez Gene: 10055 Human
- Entrez Gene: 56459 Mouse
- Entrez Gene: 308384 Rat
- Omim: 613294 Human
- SwissProt: A2VE14 Cow
- SwissProt: Q9UBE0 Human
see all -
Alternative names
- Activator of SUMO1 antibody
- AOS1 antibody
- HSPC140 antibody
see all
Images
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All lanes : Anti-SAE1 (phospho S185) antibody (ab106096) at 1/1000 dilution
Lane 1 : HeLa whole cell protein
Lane 2 : HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated secondary
Developed using the ECL technique.
Predicted band size: 38 kDa
10% SDS-PAGE. Lower panel shows tubulin loading control
Datasheets and documents
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Datasheet download
References (0)
ab106096 has not yet been referenced specifically in any publications.