Anti-SAR1 antibody (ab125871)
Key features and details
- Rabbit polyclonal to SAR1
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SAR1 antibody -
Description
Rabbit polyclonal to SAR1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Cow, Pig, Xenopus laevis, Zebrafish -
Immunogen
Recombinant fragment, corresponding to a region within N terminal amino acids 6-180 of Human SAR1 (Q9NR31).
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Positive control
- 293T and A431 whole cell lysates; A431 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab125871 is purified by antigen-affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab125871 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 22 kDa.
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ICC/IF |
1/100 - 1/500.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 22 kDa. |
ICC/IF
1/100 - 1/500. |
Target
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Function
Involved in transport from the endoplasmic reticulum to the Golgi apparatus (By similarity). Required to maintain SEC16A localization at discrete locations on the ER membrane perhaps by preventing its dissociation. SAR1A-GTP-dependent assembly of SEC16A on the ER membrane forms an organized scaffold defining endoplasmic reticulum exit sites (ERES). -
Sequence similarities
Belongs to the small GTPase superfamily. SAR1 family. -
Cellular localization
Endoplasmic reticulum. Golgi apparatus. - Information by UniProt
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Database links
- Entrez Gene: 56681 Human
- Entrez Gene: 20224 Mouse
- Entrez Gene: 361842 Rat
- Omim: 607691 Human
- SwissProt: Q9NR31 Human
- SwissProt: P36536 Mouse
- Unigene: 499960 Human
- Unigene: 594910 Human
see all -
Alternative names
- COPII associated small GTPase antibody
- COPII-associated small GTPase antibody
- GTP binding protein SAR1a antibody
see all
Images
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All lanes : Anti-SAR1 antibody (ab125871) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
12% SDS PAGE -
ab125871, at 1/500, staining SAR1 in methanol-fixed A431 cells by Immunofluorescence. Lower image is co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab125871 has been referenced in 3 publications.
- Phuyal S et al. Mechanical strain stimulates COPII-dependent secretory trafficking via Rac1. EMBO J 41:e110596 (2022). PubMed: 35938214
- McKnight KL et al. Stimulator of interferon genes (STING) is an essential proviral host factor for human rhinovirus species A and C. Proc Natl Acad Sci U S A 117:27598-27607 (2020). PubMed: 33060297
- Vanhoutte D et al. Thrombospondin expression in myofibers stabilizes muscle membranes. Elife 5:N/A (2016). WB ; Mouse . PubMed: 27669143