Overview

  • Product name

  • Description

    Goat polyclonal to SEC23
  • Host species

    Goat
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Horse, Cow, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-ANRAATTGHVID

    , corresponding to internal sequence amino acids 335-346 of Human SEC23

  • Positive control

    • NIH 3T3 lysate

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab99552 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 86 kDa).

Target

  • Function

    Component of the COPII coat, that covers ER-derived vesicles involved in transport from the endoplasmic reticulum to the Golgi apparatus. COPII acts in the cytoplasm to promote the transport of secretory, plasma membrane, and vacuolar proteins from the endoplasmic reticulum to the Golgi complex.
  • Involvement in disease

    Defects in SEC23A are the cause of craniolenticulosutural dysplasia (CLSD) [MIM:607812]; also known as cranio-lenticulo-sutural dysplasia. CLSD is an autosomal recessive syndrome characterized by late-closing fontanels, sutural cataracts, facial dysmorphisms and skeletal defects.
  • Sequence similarities

    Belongs to the SEC23/SEC24 family. SEC23 subfamily.
  • Cellular localization

    Smooth endoplasmic reticulum membrane. Golgi apparatus membrane. In the ribosome-free transitional face of the ER and associated vesicles.
  • Information by UniProt
  • Database links

  • Alternative names

    • CLSD antibody
    • Protein transport protein Sec23A antibody
    • SC23A_HUMAN antibody
    • Sec23 homolog A (S. cerevisiae) antibody
    • SEC23-related protein A antibody
    • sec23a antibody
    see all

Images

  • Anti-SEC23 antibody (ab99552) at 2 µg/ml (incubation time 1 hour) + NIH 3T3 cell lysate in RIPA buffer at 35 µg

    Developed using the ECL technique.

    Predicted band size: 86 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?

References

ab99552 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293 A549 Cos7 U2OS)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
HEK293 A549 Cos7 U2OS
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Aug 01 2018

Answer

Thank you for contacting us. Because we carry over 95,000 products, it isn't feasible for us to keep small sample sizes of our products.

We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.

If the product is to be used in an untested species or application, you may be eligible for our testing discount program if the antibody has not yet been purchased. Please contact our Scientific Support team by replying to this email prior to purchase for more information.

Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.

To find out more about our Abreview system, please see the following link:

https://www.abcam.com/abreviews



I hope this information is helpful. Please do not hesitate to contact us again with any other questions.

Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Panc-1)
Specification
Panc-1
Fixative
Formaldehyde
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C

Dr. Armen Petrosyan

Verified customer

Submitted Apr 28 2011

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