Anti-SEL1L antibody (ab78298)
Key features and details
- Rabbit polyclonal to SEL1L
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEL1L antibody -
Description
Rabbit polyclonal to SEL1L -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Hamster -
Immunogen
Synthetic peptide corresponding to Human SEL1L aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab86659) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab78298 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa).
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753). |
Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 89 kDa). SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753). |
Target
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Function
May play a role in Notch signaling (By similarity). May be involved in the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. -
Tissue specificity
Highly expressed in pancreas. -
Sequence similarities
Belongs to the sel-1 family.
Contains 1 fibronectin type-II domain.
Contains 11 Sel1-like repeats. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6400 Human
- Entrez Gene: 20338 Mouse
- Entrez Gene: 116488 Rat
- Entrez Gene: 314352 Rat
- Omim: 602329 Human
- SwissProt: Q12916 Human
- SwissProt: Q9UBV2 Human
- SwissProt: Q9Z2G6 Mouse
see all -
Alternative names
- IBD2 antibody
- PRO1063 antibody
- Protein sel-1 homolog 1 antibody
see all
Images
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Anti-SEL1L antibody (ab78298) at 1 µg/ml + HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 89 kDa
Observed band size: 100 kDa why is the actual band size different from the predicted?
Exposure time: 4 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab78298 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
SEL1L has a predicted molecular weight of 89 kDa; however it has a number of potential glycosylation sites which may affect the migration of the protein (SwissProt data). This migration has been observed in the literature (PMID:18502753).
Datasheets and documents
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SDS download
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Datasheet download
References (23)
ab78298 has been referenced in 23 publications.
- Bhattacharya A et al. SEL1L-HRD1 ER-associated degradation suppresses hepatocyte hyperproliferation and liver cancer. iScience 25:105183 (2022). PubMed: 36238898
- Yoshida S et al. Endoplasmic reticulum-associated degradation is required for nephrin maturation and kidney glomerular filtration function. J Clin Invest 131:N/A (2021). PubMed: 33591954
- Liu X et al. Notch-induced endoplasmic reticulum-associated degradation governs mouse thymocyte β-selection. Elife 10:N/A (2021). PubMed: 34240701
- Ibe NU et al. Non-canonical activation of the ER stress sensor ATF6 by Legionella pneumophila effectors. Life Sci Alliance 4:N/A (2021). PubMed: 34635501
- Wang Y et al. NEDD4L-mediated LTF protein degradation limits ferroptosis. Biochem Biophys Res Commun 531:581-587 (2020). PubMed: 32811647