This antibody gave a positive signal in Human, Mouse and Rat Skeletal Muscle tissue lysates.
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pH: 7.40 Preservative: 0.02% Sodium azide Constituent: PBS Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 110 kDa).
Key regulator of striated muscle performance by acting as the major Ca(2+) ATPase responsible for the reuptake of cytosolic Ca(2+) into the sarcoplasmic reticulum. Catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen. Contributes to calcium sequestration involved in muscular excitation/contraction.
Skeletal muscle, fast twitch muscle (type II) fibers.
Involvement in disease
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIA subfamily.
Isoform SERCA1A accounts for more than 99% of SERCA1 isoforms expressed in adult skeletal muscle, while isoform SERCA1B predominates in neo-natal skeletal muscle.
The predicted molecular weight of SERCA1 ATPase is 110 kDa (SwissProt), however we expect to observe a banding pattern at 99 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.