Anti-SERPINB1/PI2 antibody (ab47731)
Key features and details
- Rabbit polyclonal to SERPINB1/PI2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SERPINB1/PI2 antibody
See all SERPINB1/PI2 primary antibodies -
Description
Rabbit polyclonal to SERPINB1/PI2 -
Host species
Rabbit -
Specificity
This antibody is specific for SERPINB1/PI2.
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Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SERPINB1/PI2 (N terminal).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab47731 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/1000 - 1/5000. Detects a band of approximately 47 kDa (predicted molecular weight: 43 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Regulates the activity of the neutrophil proteases elastase, cathepsin G, proteinase-3, chymase, chymotrypsin, and kallikrein-3. -
Sequence similarities
Belongs to the serpin family. Ov-serpin subfamily. -
Domain
Reactive bond 1 is specific for reaction with chymotrypsin-like protease such as cathepsin G, chymotrypsin or chymase, while reactive bond 2 is specific for reaction with elastase-like protease such as neutrophyl elastase, proteinase-3, pancreatic elastase or PSA. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 1992 Human
- Omim: 130135 Human
- SwissProt: P30740 Human
- Unigene: 381167 Human
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Alternative names
- Anti elastase antibody
- EI antibody
- ELANH2 antibody
see all
Images
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All lanes : Anti-SERPINB1/PI2 antibody (ab47731) at 1/1000 dilution
All lanes : MH-S cell lysate
Lysates/proteins at 20 µl per lane.
Predicted band size: 43 kDa
Observed band size: 47 kDa why is the actual band size different from the predicted?
Additional bands at: 65 kDa (possible degradation product) -
ab47731 (2µg/ml) staining Serpin B1 in human spleen using an automated system (DAKO Autostainer Plus). Using this protocol there is cytoplasmic staining in both red and white pulp.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab47731 has been referenced in 5 publications.
- Sommeregger W et al. Proteomic differences in recombinant CHO cells producing two similar antibody fragments. Biotechnol Bioeng 113:1902-12 (2016). WB . PubMed: 26913574
- van Kempen PM et al. Oropharyngeal squamous cell carcinomas differentially express granzyme inhibitors. Cancer Immunol Immunother 65:575-85 (2016). PubMed: 26993499
- Vermeulen JF et al. Pediatric Primitive Neuroectodermal Tumors of the Central Nervous System Differentially Express Granzyme Inhibitors. PLoS One 11:e0151465 (2016). PubMed: 26963506
- Uchiyama K et al. Serpin B1 protects colonic epithelial cell via blockage of neutrophil elastase activity and its expression is enhanced in patients with ulcerative colitis. Am J Physiol Gastrointest Liver Physiol 302:G1163-70 (2012). WB, IHC-P ; Human . PubMed: 22421620
- Tseng MY et al. Serine protease inhibitor (SERPIN) B1 promotes oral cancer cell motility and is over-expressed in invasive oral squamous cell carcinoma. Oral Oncol 45:771-6 (2009). WB, IHC-P ; Human . PubMed: 19213596