Overview

  • Product name

  • Description

    Rabbit polyclonal to SF3B14
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthesized peptide derived from the C-terminal region of human SF3B14

  • Positive control

    • Extracts from HepG2 cells This antibody gave a positive result when used in the following methanol fixed cell lines: HepG2.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74676 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 14 kDa.
ELISA 1/40000.
ICC/IF Use a concentration of 5 µg/ml.

Target

  • Function

    Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively.
  • Sequence similarities

    Contains 1 RRM (RNA recognition motif) domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Ht006 antibody
    • P14 antibody
    • PM14_HUMAN antibody
    • Pre mRNA branch site protein p14 antibody
    • Pre-mRNA branch site protein p14 antibody
    • SAP14 antibody
    • SF3B 14 kDa subunit antibody
    • SF3B14 antibody
    • SF3B14a antibody
    • Splicing factor 3B, 14 kDa subunit antibody
    see all

Images

  • ICC/IF image of ab74676 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab74676 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-SF3B14 antibody (ab74676) at 1/500 dilution

    Lane 1 : extracts from HepG2 cells
    Lane 2 : extracts from HepG2 cells with immunizing peptide at 10 µg

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 14 kDa
    Observed band size: 19 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa. We are unsure as to the identity of these extra bands.

References

ab74676 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

I am sorry that these antibodies did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department. Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department. The credit note ID is for your reference only and does not automatically guarantee the credit. I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

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