Our Abpromise guarantee covers the use of ab4193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
ICC/IF Use a concentration of 5 µg/ml.
IHC-P 1/800. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Boil the sections for 20-30 minutes in water bath. We also recomend trying different antigen retrieval methods with different buffers if one does not work.

WB 1/230. Predicted molecular weight: 37 kDa.


  • Function
    Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels (By similarity). Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase (By similarity). In kidney development, inhibits tubule formation and bud growth in metanephroi (By similarity). Inhibits WNT1/WNT4-mediated TCF-dependent transcription.
  • Tissue specificity
    Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes.
  • Sequence similarities
    Belongs to the secreted frizzled-related protein (sFRP) family.
    Contains 1 FZ (frizzled) domain.
    Contains 1 NTR domain.
  • Domain
    The FZ domain is involved in binding with Wnt ligands.
  • Cellular localization
    Secreted. Cell membrane or extracellular matrix-associated. Released by heparin-binding.
  • Information by UniProt
  • Database links
  • Alternative names
    • Frizzled related protein 1 antibody
    • FRP 1 antibody
    • FRP antibody
    • FRP-1 antibody
    • FRP1 antibody
    • FrzA antibody
    • SARP 2 antibody
    • SARP-2 antibody
    • SARP2 antibody
    • Secreted apoptosis related protein 2 antibody
    • Secreted apoptosis-related protein 2 antibody
    • Secreted frizzled related protein 1 antibody
    • Secreted frizzled related protein antibody
    • Secreted frizzled-related protein 1 antibody
    • SFRP 1 antibody
    • sFRP-1 antibody
    • SFRP1 antibody
    • SFRP1_HUMAN antibody
    see all


  • ab4193 staining human dermal hypertrophic scar tissue sections by IHC-P.  Sections were formaldehyde fixed and subjected to heat mediated antigen retrieval prior to blocking (with a commercial protein block) and incubating with the primary antibody (diluted 1/800) for 20 minutes.  A HRP-conjugated goat anti-rabbit antibody was used as the secondary.

    See Abreview

  • Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.

    Expected band size: 37kD.

    Western blot using ab4193 at 1/230 (o/n 4°C) on lysates from cultured small airway epithelial cells.

  • ICC/IF image of ab4193 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab4193, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab4193 staining SFRP1 by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in human breast cancer cells, injected subcutaneously into mice and grown until the tumour was a palpable size. Tissue underwent paraformaldehyde fixation before heat mediated antigen retrieval in 10mM sodium citrate- EDTA (pH 6). The primary antibody was diluted 1/1000 and incubated with sample for 17 hours at 4°C in 10% Casein. A Biotin conjugated goat polyclonal to rabbit IgG was used at dilution at 1/200 as secondary antibody.

    See Abreview

  • ab4193 detecting SFRP1 in Human Tear samples (purified protein) by sandwich ELISA. Protein was captured and Samples were blocked with 3% milk for 2 hours at 25°C and incubated with ab4193 (1/1000 in PBS ) for 1 hour at 37°C. An alkaline phosphatase Goat anti-rabbit IgG polyclonal (1/15000) was used as the secondary antibody.

    See Abreview


This product has been referenced in:
  • Hawkshaw NJ  et al. Identifying novel strategies for treating human hair loss disorders: Cyclosporine A suppresses the Wnt inhibitor, SFRP1, in the dermal papilla of human scalp hair follicles. PLoS Biol 16:e2003705 (2018). Read more (PubMed: 29738529) »
  • Kong LY  et al. In vivo and in vitro effects of microRNA-27a on proliferation, migration and invasion of breast cancer cells through targeting of SFRP1 gene via Wnt/ß-catenin signaling pathway. Oncotarget 8:15507-15519 (2017). WB ; Human . Read more (PubMed: 28099945) »

See all 25 Publications for this product

Customer reviews and Q&As

Your protocol appears to be correct. The only IHC data we have is from customers who used citrate heat-mediated retrieval and dilutions of 1/1000 or 1/800. The samples were hypertrophic scar and a subcutaneous implant of MDA-MB 436 breast cancer cells....

Read More

The highest expression is in the heart and kidney. For an example of a strong stain of heart muscle tissue, please see the image on the datasheet for a different SFRP1 antibody, ab126613:

Click here (or use the following: https://www.abcam.c...

Read More

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital ...

Read More

Thank you very much for providing that information. It has been very helpful in understanding the results you have seen so far in your experiment.

I am sorry but Kate is currently away from the office but I will try my best to help you with th...

Read More

Thank you for taking the time to reply with the information.

The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

I would like to r...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that our antibodies are tested and covered by our 6 month guarantee for ...

Read More

Este es un mail de confirmación del nuevo pedido, consistente en el reemplazo completamente gratuito, del anticuerpo ab4193 con número de pedido XXX.

Os llegará un mail de confirmación del nuevo pedido, si necesit&...

Read More

Gracias por contactarnos y alertarnos del problema sufrido con este anticuerpo.

Efectivamente los resultados obtenidos con este nuevo lote no se corresponden con los esperados, y le pido disculpas por las molestias que esto haya podido ocasio...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Human Purified protein (Tears)
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Sandwich (Detection)

Abcam user community

Verified customer

Submitted Aug 24 2012

Thank you for your email.

I apologize for the delay as it took a while to get back to you. Unfortunately the publication team at Abcam is unable to trace back the antibodies catalogue number the author have used. They can only trace one abID w...

Read More

1-10 of 19 Abreviews or Q&A


Sign up