Biotin Anti-SIRP alpha antibody [OX-41] (ab33987)
Key features and details
- Biotin Mouse monoclonal [OX-41] to SIRP alpha
- Suitable for: IHC-Fr
- Reacts with: Rat
- Conjugation: Biotin
- Isotype: IgG2a
Overview
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Product name
Biotin Anti-SIRP alpha antibody [OX-41]
See all SIRP alpha primary antibodies -
Description
Biotin Mouse monoclonal [OX-41] to SIRP alpha -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: IHC-Frmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat SIRP alpha. Peritoneal macrophages (Rat)
Database link: P97710 -
Epitope
This clone binds to a different epitope on CD172a than ED 9. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-41 -
Isotype
IgG2a -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-Fr |
Notes |
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IHC-Fr: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution. This product requires protein digestion pre-treatment of paraffin sections, eg.trypsin or pronase.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function (By similarity). Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. -
Tissue specificity
Ubiquitous. Highly expressed in brain. Detected on myeloid cells, but not T-cells. Detected at lower levels in heart, placenta, lung, testis, ovary, colon, liver, small intestine, prostate, spleen, kidney, skeletal muscle and pancreas. -
Sequence similarities
Contains 2 Ig-like C1-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Post-translational
modificationsN-glycosylated.
Phosphorylated on tyrosine residues in response to stimulation with EGF, growth hormone, insulin and PDGF. Dephosphorylated by PTPN11. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 25528 Rat
- SwissProt: P97710 Rat
- Unigene: 53971 Rat
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Alternative names
- Signal regulatory protein alpha type 1 antibody
- Bit antibody
- Brain Ig like molecule with tyrosine based activation motifs antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (2)
ab33987 has been referenced in 2 publications.
- Adams S et al. Signal-regulatory protein is selectively expressed by myeloid and neuronal cells. J Immunol 161:1853-9 (1998). PubMed: 9712053
- Robinson AP et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3. Immunology 57:239-47 (1986). PubMed: 3512425