Anti-SIRT4 antibody (ab124521)
Key features and details
- Rabbit polyclonal to SIRT4
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-SIRT4 antibody
See all SIRT4 primary antibodies -
Description
Rabbit polyclonal to SIRT4 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Synthetic peptide surrounding amino acid 72 of Rat SIRT4.
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Positive control
- Rat kidney tissue lysate
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General notes
This product is manufactured by BioVision, an Abcam company and was previously called 3224 SIRT4 Antibody. 3224-100 is the same size as the 100 µg size of ab124521.
The antibody solution should be gently mixed before use.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 69% PBS, 0.5% BSA, 30% Glycerol (glycerin, glycerine), 0.15% EDTA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124521 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 32 kDa.
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Notes |
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WB
Use a concentration of 1 - 4 µg/ml. Predicted molecular weight: 32 kDa. |
Target
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Function
Acts as NAD-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. Catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. Inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner (PubMed:25525879). Catalyzes the transfer of ADP-ribosyl groups onto target proteins, including mitochondrial GLUD1, inhibiting GLUD1 enzyme activity. Acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1: expressed in response to DNA damage and negatively regulates anaplerosis by inhibiting GLUD1, leading to block metabolism of glutamine into tricarboxylic acid cycle and promoting cell cycle arrest (PubMed:16959573, PubMed:17715127). In response to mTORC1 signal, SIRT4 expression is repressed, promoting anaplerosis and cell proliferation. Acts as a tumor suppressor (PubMed:23562301, PubMed:23663782). Also acts as a NAD-dependent protein deacetylase: mediates deacetylation of 'Lys-471' of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis (By similarity). Controls fatty acid oxidation by inhibiting PPARA transcriptional activation. Impairs SIRT1:PPARA interaction probably through the regulation of NAD(+) levels (PubMed:24043310). Down-regulates insulin secretion. -
Tissue specificity
Detected in vascular smooth muscle and striated muscle. Detected in insulin-producing beta-cells in pancreas islets of Langerhans (at protein level). Widely expressed. Weakly expressed in leukocytes and fetal thymus. -
Sequence similarities
Belongs to the sirtuin family. Class II subfamily.
Contains 1 deacetylase sirtuin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
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Alternative names
- MGC130046 antibody
- MGC130047 antibody
- MGC57437 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab124521 has been referenced in 10 publications.
- Zhang P et al. Paeoniflorin Enhances the Sensitivity of ER-Positive Breast Cancer Cells to Tamoxifen through Promoting Sirtuin 4. Evid Based Complement Alternat Med 2022:6730559 (2022). PubMed: 35154350
- Wei W et al. SIRT4 Is Highly Expressed in Retinal Müller Glial Cells. Front Neurosci 16:840443 (2022). PubMed: 35185463
- Meng L et al. Dysregulation of the Sirt5/IDH2 axis contributes to sunitinib resistance in human renal cancer cells. FEBS Open Bio 11:921-931 (2021). PubMed: 33455080
- Thangaraj A et al. HIV TAT-mediated microglial senescence: Role of SIRT3-dependent mitochondrial oxidative stress. Redox Biol 40:101843 (2021). PubMed: 33385630
- Chen Z et al. Toll-Like Receptor 4 Mediated Oxidized Low-Density Lipoprotein-Induced Foam Cell Formation in Vascular Smooth Muscle Cells via Src and Sirt1/3 Pathway. Mediators Inflamm 2021:6639252 (2021). PubMed: 33927570
- Lin W et al. Sirtuin4 suppresses the anti-neuroinflammatory activity of infiltrating regulatory T cells in the traumatically injured spinal cord. Immunology 158:362-374 (2019). PubMed: 31559637
- Fukushima A et al. Acetylation contributes to hypertrophy-caused maturational delay of cardiac energy metabolism. JCI Insight 3:N/A (2018). PubMed: 29769443
- Hu JQ et al. Histone deacetylase SIRT6 regulates chemosensitivity in liver cancer cells via modulation of FOXO3 activity. Oncol Rep 40:3635-3644 (2018). PubMed: 30542728
- Karvinen S et al. Effects of intrinsic aerobic capacity, aging and voluntary running on skeletal muscle sirtuins and heat shock proteins. Exp Gerontol 79:46-54 (2016). PubMed: 27038700
- Tsai S et al. Muscle-specific 4E-BP1 signaling activation improves metabolic parameters during aging and obesity. J Clin Invest 125:2952-64 (2015). PubMed: 26121750