Anti-SLC12A3 antibody (ab95302)
Key features and details
- Rabbit polyclonal to SLC12A3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SLC12A3 antibody
See all SLC12A3 primary antibodies -
Description
Rabbit polyclonal to SLC12A3 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide:
PGEPRKVRPTLADLHSFLKQEG
, corresponding to N terminal amino acids 74-95 of Rat SLC12A3 (NP_062218) -
Positive control
- Rat kidney tissue lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.007% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95302 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/200.
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WB |
1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
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Notes |
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ICC/IF
1/200. |
WB
1/1000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa). |
Target
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Function
Electrically silent transporter system. Mediates sodium and chloride reabsorption. -
Tissue specificity
Predominant in kidney. -
Involvement in disease
Defects in SLC12A3 are the cause of Gitelman syndrome (GS) [MIM:263800]. GS is an autosomal recessive disorder characterized by hypokalemic alkalosis in combination with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. Patients are often asymptomatic or present transient periods of muscular weakness and tetany, usually accompanied by abdominal pain, vomiting and fever. The phenotype is highly heterogeneous in terms of age at onset and severity. Cardinal features such as hypocalciuria and hypomagnesemia might also change during the life cycle of a given patient. GS has overlapping features with Bartter syndrome. -
Sequence similarities
Belongs to the SLC12A transporter family. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 6559 Human
- Entrez Gene: 20497 Mouse
- Entrez Gene: 54300 Rat
- Omim: 600968 Human
- SwissProt: P55017 Human
- SwissProt: P59158 Mouse
- SwissProt: P55018 Rat
- Unigene: 669115 Human
see all -
Alternative names
- FLJ96318 antibody
- Na Cl cotransporter antibody
- Na Cl symporter antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab95302 has been referenced in 6 publications.
- Hanai S et al. Hypoxia-induced thyroid hormone receptor expression regulates cell-cycle progression in renal tubule epithelial cells. Endocr J 68:1309-1320 (2021). PubMed: 34108302
- Jobbagy S et al. Nrf2 activation protects against lithium-induced nephrogenic diabetes insipidus. JCI Insight 5:N/A (2020). PubMed: 31941842
- Wang LJ et al. PGF2a stimulates the 10-pS Cl- channel and thiazide-sensitive Na+-Cl- cotransporter in the distal convoluted tubule. Am J Physiol Renal Physiol 319:F414-F422 (2020). PubMed: 32715760
- Tang TT et al. Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI. Sci Adv 6:eaaz0748 (2020). PubMed: 32851154
- Zhang J et al. Critical role of the SPAK protein kinase CCT domain in controlling blood pressure. Hum Mol Genet 24:4545-58 (2015). PubMed: 25994507
- Schumacher FR et al. Characterisation of the Cullin-3 mutation that causes a severe form of familial hypertension and hyperkalaemia. EMBO Mol Med 7:1285-306 (2015). PubMed: 26286618