Recombinant
RabMAb

Recombinant Anti-SOCS7 antibody [EPR8466] (ab133677)

Overview

  • Product name
    Anti-SOCS7 antibody [EPR8466]
    See all SOCS7 primary antibodies
  • Description
    Rabbit monoclonal [EPR8466] to SOCS7
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SOCS7 (N terminal). The exact sequence is proprietary.

  • Positive control
    • MCF7, HeLa, Jurkat and U-87 MG cell lysates; Human brain tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)
    KD = 1.07 x 10 -10 M
    Learn more about KD
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR8466
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab133677 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 63 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function
      Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
    • Tissue specificity
      Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain.
    • Pathway
      Protein modification; protein ubiquitination.
    • Sequence similarities
      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • Domain
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). It is required for IRS1 ubiquitination and subsequent proteasomal degradation.
    • Cellular localization
      Cytoplasm. Cell membrane. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence.
    • Information by UniProt
    • Database links
    • Alternative names
      • Ash and phospholipase C gamma-binding protein antibody
      • NAP 4 antibody
      • NAP 4 Fragment antibody
      • NAP-4 antibody
      • NAP4 antibody
      • Nck antibody
      • Nck Ash and phospholipase C binding protein antibody
      • Nck Ash and phospholipase C gamma binding protein antibody
      • Nck associated protein 4 antibody
      • Nck-associated protein 4 antibody
      • NCKAP4 antibody
      • SH2 domain containing SOCS box protein antibody
      • SOCS 7 antibody
      • SOCS-7 antibody
      • SOCS4 antibody
      • SOCS6 antibody
      • Socs7 antibody
      • SOCS7_HUMAN antibody
      • Suppressor of cytokine signaling 7 antibody
      see all

    Images

    • All lanes : Anti-SOCS7 antibody [EPR8466] (ab133677) at 1/1000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : U-87 MG cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 63 kDa

    • Immunohistochemical analysis of paraffin-embedded Human brain tissue labelling SOCS7 with ab133677 at 1/100 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133677 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab133677.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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