Anti-SOCS7 antibody (ab224589)
Key features and details
- Rabbit polyclonal to SOCS7
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-SOCS7 antibody
See all SOCS7 primary antibodies -
Description
Rabbit polyclonal to SOCS7 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Recombinant fragment corresponding to Human SOCS7 aa 6-165.
Database link: O14512 -
Positive control
- WB: MCF7, HeLa, Jurkat and U-87 MG cell lysate; rat heart and liver tissue lysate. ICC/IF: HepG2 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Constituents: 40% Glycerol (glycerin, glycerine), PBS, 0.03% Proclin 300 -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224589 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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WB |
1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa).
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/500 - 1/5000. Detects a band of approximately 63 kDa (predicted molecular weight: 63 kDa). |
Target
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Function
Regulates signaling cascades probably through protein ubiquitination and/or sequestration. Functions in insulin signaling and glucose homeostasis through IRS1 ubiquitination and subsequent proteasomal degradation. Inhibits also prolactin, growth hormone and leptin signaling by preventing STAT3 and STAT5 activation, sequestering them in the cytoplasm and reducing their binding to DNA. May be a substrate recognition component of a SCF-like E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. -
Tissue specificity
Expressed in brain and leukocytes. Also in fetal lung fibroblasts and fetal brain. -
Pathway
Protein modification; protein ubiquitination. -
Sequence similarities
Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
Domain
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes (By similarity). It is required for IRS1 ubiquitination and subsequent proteasomal degradation. -
Cellular localization
Cytoplasm. Cell membrane. Nucleus. Mostly cytoplasmic, but shuttles between the cytoplasm and the nucleus. Rapidly relocalizes to the nucleus after UV irradiation. Cytoplasmic location depends upon SEPT7 presence. - Information by UniProt
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Database links
- Entrez Gene: 30837 Human
- Entrez Gene: 287659 Rat
- Omim: 608788 Human
- SwissProt: O14512 Human
- Unigene: 514132 Human
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Alternative names
- Ash and phospholipase C gamma-binding protein antibody
- NAP 4 antibody
- NAP 4 Fragment antibody
see all
Images
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All lanes : Anti-SOCS7 antibody (ab224589) at 1/500 dilution
Lane 1 : MCF7 (human breast adenocarcinoma cell line) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate
Lane 4 : U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Lane 5 : Rat heart tissue lysate
Lane 6 : Rat liver tissue lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 63 kDa
Observed band size: 63 kDa -
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for SOCS7 (green) using ab224589 at 1/100 dilution in ICC/IF.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab224589 has been referenced in 4 publications.
- Du Y et al. SOCS7/HuR/FOXM1 signaling axis inhibited high-grade serous ovarian carcinoma progression. J Exp Clin Cancer Res 41:185 (2022). PubMed: 35624501
- Wang C et al. Coiled-Coil Domain-Containing 68 Downregulation Promotes Colorectal Cancer Cell Growth by Inhibiting ITCH-Mediated CDK4 Degradation. Front Oncol 11:668743 (2021). PubMed: 33968776
- Ding R et al. DZNep, an inhibitor of the histone methyltransferase EZH2, suppresses hepatic fibrosis through regulating miR-199a-5p/SOCS7 pathway. PeerJ 9:e11374 (2021). PubMed: 34040893
- Su M et al. miR-885-5p upregulation promotes colorectal cancer cell proliferation and migration by targeting suppressor of cytokine signaling. Oncol Lett 16:65-72 (2018). WB ; Human . PubMed: 29928388