Overview

  • Product name
    Anti-SOX2 (GlcNAc S248) antibody
    See all SOX2 primary antibodies
  • Description
    Rabbit polyclonal to SOX2 (GlcNAc S248)
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse SOX2 aa 200-300 (glcnac S248) conjugated to Keyhole Limpet Haemocyanin (KLH).
    (Peptide available as ab157602, ab157602)

  • Positive control
    • This antibody gave a positive signal in E14Tg2A and IOUD2 whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab136167 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 34 kDa).

Target

  • Function
    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease
    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications
    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Images

  • All lanes : Anti-SOX2 (GlcNAc S248) antibody (ab136167) at 1 µg/ml

    Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 2 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate
    Lane 3 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
    Lane 4 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with SOX2 (glcnac S248) peptide (ab157602) at 1 µg/ml
    Lane 5 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml
    Lane 6 : IOUD2 (Mouse embryonic stem cell line) Whole Cell Lysate with Mouse SOX2 peptide (ab157771) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 34 kDa
    Observed band size: 40 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 148 kDa, 24 kDa, 29 kDa, 62 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 1 minute


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab136167 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

This product has been referenced in:
  • Speakman CM  et al. Elevated O-GlcNAc levels activate epigenetically repressed genes and delay mouse ES cell differentiation without affecting naïve to primed cell transition. Stem Cells N/A:N/A (2014). WB ; Mouse . Read more (PubMed: 24898611) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (4-12% Nupage, MOPS)
Sample
Mouse Purified protein (Immunoprecipitated from 100ug of whole cell lysate)
Specification
Immunoprecipitated from 100ug of whole cell lysate
Treatment
DMSO or GlcNAcstatin C (Oga inhibitor) for 24h
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Apr 04 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Embryonic stem cells (E14Tg2a))
Loading amount
10 µg
Specification
Embryonic stem cells (E14Tg2a)
Treatment
Vehicle (DMSO) 1/1000, GlcNAcstatin C (1microM in DMSO), 4Ac5SGlcNAc (50microM in DMSO) all 16h
Gel Running Conditions
Reduced Denaturing (4-12% Nupage, MOPS)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Feb 28 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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