Product nameAnti-SOX6 antibody
See all SOX6 primary antibodies
DescriptionRabbit polyclonal to SOX6
Tested applicationsSuitable for: WB, IHC-Frmore details
Species reactivityReacts with: Mouse, Cow, Human
Predicted to work with: Rat, Horse, Non human primates
- This antibody gave a positive signal in HeLa and Jurkat Nuclear Extracts.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferConstituents: PBS, 5% Trehalose
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab66316 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 93 kDa (predicted molecular weight: 93 kDa).|
|IHC-Fr||Use at an assay dependent concentration. PubMed: 21880783|
FunctionTranscriptional activator. Binds specifically to the DNA sequence 5'-AACAAT-3'. Plays a key role in several developmental processes, including neurogenesis and skeleton formation.
Tissue specificityExpressed in a wide variety of tissues, most abundantly in skeletal muscle.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
modificationsSumoylation inhibits the transcriptional activity.
- Information by UniProt
- HSSOX 6 antibody
- HSSOX6 antibody
- SOX 6 antibody
This image shows notochord of 12 d.p.c. mouse embryos stained with ab66316 at 1/100 dilution. Detection was done using anti-rabbit-Cy3 conjugated secondary antibodies (red fluorescence). Cell nuceli were counterstained with DAPI (blue fluorescence).
All lanes : Anti-SOX6 antibody (ab66316) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 :
Jurkat nuclear extract lysate (ab14844)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 93 kDa
Observed band size: 93 kDa
Additional bands at: 20 kDa, 50 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 150 seconds
This product has been referenced in:
- Ahmed N et al. Serum- and growth-factor-free three-dimensional culture system supports cartilage tissue formation by promoting collagen synthesis via Sox9-Col2a1 interaction. Tissue Eng Part A 20:2224-33 (2014). WB ; Cow . Read more (PubMed: 24606204) »
- Wang X et al. Prdm1a and miR-499 act sequentially to restrict Sox6 activity to the fast-twitch muscle lineage in the zebrafish embryo. Development 138:4399-404 (2011). IHC-Fr ; Zebrafish . Read more (PubMed: 21880783) »