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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box
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RecombinantRabMAb

Recombinant Anti-SOX9 antibody [EPR14335-78] (ab185966)

  • Datasheet
  • SDS
  • Certificate of Compliance
Reviews (24) Submit a question References (58)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
  • Anti-SOX9 antibody [EPR14335-78] (ab185966)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14335-78] to SOX9
  • Suitable for: Flow Cyt, ICC/IF, WB, IHC-P
  • Reacts with: Mouse, Rat, Human

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View more associated products

Overview

  • Product name

    Anti-SOX9 antibody [EPR14335-78]
    See all SOX9 primary antibodies
  • Description

    Rabbit monoclonal [EPR14335-78] to SOX9
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Mouse
    Rat
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse eye tissue. Rat, mouse and human colon tissue. Human breast carcinoma tissue. WB: SW480 and PC-3 cell lysate. ICC/IF: SW480, F9 and PC-3 cells. Flow Cyt: PC-3 cells.
  • General notes

      

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14335-78
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Stem Cells
    • Neural Stem Cells
    • Intracellular
    • Stem Cells
    • Mesenchymal Stem Cells
    • Chondrogenesis
    • Stem Cells
    • Neural Stem Cells
    • Neural Crest Stem Cells
    • Developmental Biology
    • Reproduction
    • Sex determination
    • Developmental Biology
    • Reproduction
    • Placental development
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Developmental Biology
    • Organogenesis
    • Skeletal development
    • Bone

Associated products

  • Alternative Versions

    • Alexa Fluor® 647 Anti-SOX9 antibody [EPR14335-78] (ab207677)
    • Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335-78] (ab208427)
    • PE Anti-SOX9 antibody [EPR14335-78] (ab224019)
    • Anti-SOX9 antibody [EPR14335-78] - BSA and Azide free (ab225541)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • SW480 whole cell lysate (ab3957)
  • Recombinant Protein

    • Recombinant Human SOX9 protein (ab131911)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Recombinant Human SOX9 protein (ab131911)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
Flow Cyt
Human
ICC/IF
Mouse
Human
IHC-P
Mouse
Rat
Human
WB
Human
All applications
Pig
Application Abreviews Notes
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF (5)
Use a concentration of 5 µg/ml.
WB (4)
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P (5)
1/1000.

Perform heat mediated antigen retrieval with citrate buffer.

Notes
Flow Cyt
Use at an assay dependent concentration.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF
Use a concentration of 5 µg/ml.
WB
1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P
1/1000.

Perform heat mediated antigen retrieval with citrate buffer.

Target

  • Function

    Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
  • Involvement in disease

    Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession P48436 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6662 Human
    • Entrez Gene: 20682 Mouse
    • Entrez Gene: 396840 Pig
    • Entrez Gene: 140586 Rat
    • Omim: 608160 Human
    • SwissProt: P48436 Human
    • SwissProt: Q04887 Mouse
    • SwissProt: O18896 Pig
    • Unigene: 647409 Human
    • Unigene: 286407 Mouse
    • Unigene: 95086 Rat
    see all
  • Alternative names

    • campomelic dysplasia autosomal sex reversal antibody
    • CMD 1 antibody
    • CMD1 antibody
    • CMPD 1 antibody
    • CMPD1 antibody
    • SOX 9 antibody
    • Sox9 antibody
    • SOX9_HUMAN antibody
    • SRA 1 antibody
    • SRA1 antibody
    • SRXX2 antibody
    • SRXY10 antibody
    • SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
    • SRY (sex determining region Y) box 9 antibody
    • SRY (sex determining region Y)-box 9 antibody
    • SRY (sex-determining region Y)-box 9 protein antibody
    • SRY related HMG box gene 9 antibody
    • Transcription factor SOX 9 antibody
    • Transcription factor SOX-9 antibody
    • transcription factor SOX9 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    ab185966 staining SOX9 in developing eye of mouse tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/1000 in PBS) at 21°C for 4 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/20000 dilution + SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    ab185966 staining Sox9 in F9 (Mouse embryonic testicular cancer cell line) cells.

    The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5 μg/ml concentration and ab7291, mouse monoclonal [DM1A] to alpha Tubulin, at  1μg/ml concentration, followed by a further incubation at room temperature for 1 h with an anti-rabbit AlexaFluor® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/5000 dilution + PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 56 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunohistochemistry analysis of paraffin-embedded rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunofluorescence analysis of 4% paraformaldehyde fixed PC-3 (Human prostate adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution (Left panel, red).

    Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. DAPI staining (Right panel, blue).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunohistochemistry analysis of paraffin-embedded mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunohistochemistry analysis of paraffin-embedded human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

    Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded pig small intestine sections labeling SOX9 with ab185966 at dilution of 1/2000.

    The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

    The image shows intense enterocyte/goblet cell nuclear positivity, confined to the crypts of Lieberkühn.

    See Abreview

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

    Immunofluorescence analysis of 4% paraformaldehyde fixed SW480 (Human colorectal adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. DAPI staining shown in blue.

  • Anti-SOX9 antibody [EPR14335-78] (ab185966)
    Anti-SOX9 antibody [EPR14335-78] (ab185966)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (58)

Publishing research using ab185966? Please let us know so that we can cite the reference in this datasheet.

ab185966 has been referenced in 58 publications.

  • Lee JK  et al. Anterior cruciate ligament remnant cells have different potentials for cell differentiation based on their location. Sci Rep 10:3097 (2020). PubMed: 32080322
  • Ma C  et al. The pro-inflammatory effect of NR4A3 in osteoarthritis. J Cell Mol Med 24:930-940 (2020). PubMed: 31701670
  • Lin JY  et al. In vitro expansion of pancreatic islet clusters facilitated by hormones and chemicals. Cell Discov 6:20 (2020). PubMed: 32284878
  • Guo J  et al. The Dynamic Transcriptional Cell Atlas of Testis Development during Human Puberty. Cell Stem Cell 26:262-276.e4 (2020). PubMed: 31928944
  • Carpinelli MR  et al. Inactivation of Zeb1 in GRHL2-deficient mouse embryos rescues mid-gestation viability and secondary palate closure. Dis Model Mech 13:N/A (2020). PubMed: 32005677
View all Publications for this product

Customer reviews and Q&As

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1-10 of 24 Abreviews or Q&A

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton X-100 1%
Specification
Cerebellum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 21 2021

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (embryo 9 weeks of gestation)
Permeabilization
Yes - Triton
Specification
embryo 9 weeks of gestation
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C
Fixative
Paraformaldehyde
Read More

Dr. Alain Chedotal

Verified customer

Submitted Jan 20 2021

IHC - Wholemount abreview for Anti-SOX9 antibody [EPR14335-78]

Average
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Mouse Embryo (E12.5 whole embryo)
Specification
E12.5 whole embryo
Read More

Dr. Alain Chedotal

Verified customer

Submitted Nov 23 2020

IHC - Wholemount abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
IHC - Wholemount
Sample
Human Embryo (whole lung (age PCW7))
Specification
whole lung (age PCW7)
Read More

Dr. Alain Chedotal

Verified customer

Submitted Nov 23 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Cerebellum)
Antigen retrieval step
None
Permeabilization
Yes - PBS 1x, Triton X-100 1%
Specification
Cerebellum
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jun 04 2020

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Tissue lysate - whole (Cerebellum)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
Cerebellum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Mar 15 2020

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Liver)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
40 µg
Specification
Liver
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 26 2020

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Gel Running Conditions
Reduced Denaturing (4-15% gradient)
Loading amount
20 µg
Specification
HEK293
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

Submitted Feb 17 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Optic nerve)
Antigen retrieval step
None
Permeabilization
Yes - TritonX-100 + Tween20
Specification
Optic nerve
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 16 2020

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Dog Tissue sections (Optic nerve)
Antigen retrieval step
None
Permeabilization
Yes - TritonX-100 + Tween20
Specification
Optic nerve
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Jan 16 2020

1-10 of 24 Abreviews or Q&A

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