Name: Anti-SOX9 antibody [EPR14335-78]
Brand: RabMAb
SKU: ab185966
Rating: 5 (19 reviews)

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Primary - Rabbit Anti-SOX9 antibody [EPR14335-78] (ab185966)
Flow Cyt, ICC/IF, WB, IHC-P

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant Human SOX9 protein (ab131911)
ELISA, WB, SDS-PAGE

View more associated products

Product name

Anti-SOX9 antibody [EPR14335-78]
See all SOX9 primary antibodies
Description

Rabbit monoclonal [EPR14335-78] to SOX9
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human, Pig
Immunogen

Recombinant fragment within Human SOX9 aa 150-300 (internal sequence). The exact sequence is proprietary.
Database link: P48436
Positive control
- IHC-P: Mouse eye tissue. Rat, mouse and human colon tissue. Human breast carcinoma tissue. WB: SW480 and PC-3 cell lysate. ICC/IF: SW480, F9 and PC-3 cells. Flow Cyt: PC-3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14335-78
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- SW480 whole cell lysate (ab3957)
Recombinant Protein
- Recombinant Human SOX9 protein (ab131911)
Related Products
- Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
- Recombinant Human SOX9 protein (ab131911)

Our Abpromise guarantee covers the use of ab185966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		Use a concentration of 5 µg/ml.
WB		1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P		1/1000. Perform heat mediated antigen retrieval with citrate buffer.

Function

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease

Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48436 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 396840 Pig
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- SwissProt: O18896 Pig
- Unigene: 647409 Human
- Unigene: 286407 Mouse
- Unigene: 95086 Rat
see all
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
- CMPD 1 antibody
- CMPD1 antibody
- SOX 9 antibody
- Sox9 antibody
- SOX9_HUMAN antibody
- SRA 1 antibody
- SRA1 antibody
- SRXX2 antibody
- SRXY10 antibody
- SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
- SRY (sex determining region Y) box 9 antibody
- SRY (sex determining region Y)-box 9 antibody
- SRY (sex-determining region Y)-box 9 protein antibody
- SRY related HMG box gene 9 antibody
- Transcription factor SOX 9 antibody
- Transcription factor SOX-9 antibody
- transcription factor SOX9 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

ab185966 staining SOX9 in developing eye of mouse tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections).

Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/1000 in PBS) at 21°C for 4 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
See Abreview
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/20000 dilution + SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

ab185966 staining Sox9 in F9 (Mouse embryonic testicular cancer cell line) cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5 μg/ml concentration and ab7291, mouse monoclonal [DM1A] to alpha Tubulin, at 1μg/ml concentration, followed by a further incubation at room temperature for 1 h with an anti-rabbit AlexaFluor^® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor^® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red).

Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/5000 dilution + PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunofluorescence analysis of 4% paraformaldehyde fixed PC-3 (Human prostate adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution (Left panel, red).

Goat anti Rabbit IgG (Alexa Fluor^®555) used as secondary antibody at 1/200 dilution. DAPI staining (Right panel, blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded pig small intestine sections labeling SOX9 with ab185966 at dilution of 1/2000.

The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

The image shows intense enterocyte/goblet cell nuclear positivity, confined to the crypts of Lieberkühn.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunofluorescence analysis of 4% paraformaldehyde fixed SW480 (Human colorectal adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor^®555) used as secondary antibody at 1/200 dilution. DAPI staining shown in blue.

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

This product has been referenced in:

Arora H et al. Subcutaneous Leydig Stem Cell Autograft: A Promising Strategy to Increase Serum Testosterone. Stem Cells Transl Med 8:58-65 (2019). Read more (PubMed: 30280521) »
Yu L et al. BMP9 stimulates joint regeneration at digit amputation wounds in mice. Nat Commun 10:424 (2019). Read more (PubMed: 30723209) »

See all 31 Publications for this product

Publishing research using ab185966? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-10 of 19 Abreviews or Q&A

Application

Western blot

Sample

Rat Tissue lysate - whole (Cerebellum)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

Cerebellum

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Mar 15 2020

Application

Western blot

Sample

Mouse Tissue lysate - whole (Liver)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

40 µg

Specification

Liver

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 26 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

HEK293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Feb 17 2020

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Optic nerve)

Antigen retrieval step

None

Permeabilization

Yes - TritonX-100 + Tween20

Specification

Optic nerve

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 16 2020

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Dog Tissue sections (Optic nerve)

Antigen retrieval step

None

Permeabilization

Yes - TritonX-100 + Tween20

Specification

Optic nerve

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jan 16 2020

Application

Immunocytochemistry/ Immunofluorescence

Sample

Goat Cell (Chondrocytes)

Permeabilization

Yes - 0.3% Triton X 00

Specification

Chondrocytes

Blocking step

2.5% Natural Goat Serum, 0.05% Tween 20, 0.1% Triton-X100 in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C

Fixative

Formaldehyde

Mr. Aman Mahajan

Verified customer

Submitted Dec 24 2019

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Goat Cell (Goat Knee Cartilage tissue)

Permeabilization

Yes - 95 degree celsius for 5 min

Specification

Goat Knee Cartilage tissue

Blocking step

2.5% Natural Goat Serum, 0.05% Tween 20, 0.1% Triton-X100 in PBS as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: RT°C

Fixative

Formaldehyde

Mr. Aman Mahajan

Verified customer

Submitted Nov 13 2019

Application

Western blot

Sample

Human Cell lysate - whole cell (Prostate cancer cells)

Gel Running Conditions

Reduced Denaturing

Loading amount

20 µg

Specification

Prostate cancer cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Jul 09 2019

Application

Immunohistochemistry (Frozen sections)

Sample

Cat Tissue sections (Optic nerve)

Permeabilization

Yes - Tween20

Specification

Optic nerve

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C

Fixative

Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 12 2019

Application

Immunocytochemistry/ Immunofluorescence

Sample

Monodelphis domestica Cell (cortex)

Permeabilization

Yes - 0,1% Triton X-100 in PBS

Specification

cortex

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 5µg/mL · Temperature: 22°C

Fixative

Paraformaldehyde

Jelena Ban

Verified customer

Submitted Mar 22 2019

1-10 of 19 Abreviews or Q&A

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For licensing inquiries, please contact partnerships@abcam.com

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

Certificate of Compliance

References

Customer reviews and Q&As

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX9 antibody [EPR14335-78]