Recombinant Anti-SOX9 antibody [EPR14335-78] (ab185966)
- Datasheet
- References (25)
- Protocols
Overview
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Product nameAnti-SOX9 antibody [EPR14335-78]
See all SOX9 primary antibodies -
DescriptionRabbit monoclonal [EPR14335-78] to SOX9
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Host speciesRabbit
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Tested applicationsSuitable for: Flow Cyt, ICC/IF, WB, IHC-Pmore details
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Species reactivityReacts with: Mouse, Rat, Human, Pig
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Immunogen
Recombinant fragment within Human SOX9 aa 150-300 (internal sequence). The exact sequence is proprietary.
Database link: P48436 -
Positive control
- IHC-P: Mouse eye tissue. Rat, mouse and human colon tissue. Human breast carcinoma tissue. WB: SW480 and PC-3 cell lysate. ICC/IF: SW480, F9 and PC-3 cells. Flow Cyt: PC-3 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR14335-78
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab185966 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| Flow Cyt | Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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| ICC/IF | Use a concentration of 5 µg/ml. | |
| WB | 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa). | |
| IHC-P | 1/1000. |
Target
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FunctionPlays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
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Involvement in diseaseDefects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
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Sequence similaritiesContains 1 HMG box DNA-binding domain.
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Cellular localizationNucleus.
- Information by UniProt
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Database links
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 396840 Pig
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- SwissProt: O18896 Pig
see all -
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Images
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-267678-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdomab185966 staining SOX9 in developing eye of mouse tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections).
Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/1000 in PBS) at 21°C for 4 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
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![Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219116-anti-sox9-antibody-epr14335-78-western-blot.jpg)
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/20000 dilution + SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
![Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-274542-anti-sox9-antibody-epr14335-78-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)ab185966 staining Sox9 in F9 (Mouse embryonic testicular cancer cell line) cells.
The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5 μg/ml concentration and ab7291, mouse monoclonal [DM1A] to alpha Tubulin, at 1μg/ml concentration, followed by a further incubation at room temperature for 1 h with an anti-rabbit AlexaFluor® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
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![Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-276587-anti-sox9-antibody-epr14335-78-flow-cytometry.jpg)
Flow Cytometry - Anti-SOX9 antibody [EPR14335-78] (ab185966)Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red).
Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
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![Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219117-anti-sox9-antibody-epr14335-78-western-blot.jpg)
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/5000 dilution + PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 56 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219115-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunohistochemistry analysis of paraffin-embedded rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219110-anti-sox9-antibody-epr14335-78-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunofluorescence analysis of 4% paraformaldehyde fixed PC-3 (Human prostate adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution (Left panel, red).
Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. DAPI staining (Right panel, blue).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219114-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunohistochemistry analysis of paraffin-embedded mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219113-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunohistochemistry analysis of paraffin-embedded human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219112-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-267987-anti-sox9-antibody-epr14335-78-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United KingdomImmunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded pig small intestine sections labeling SOX9 with ab185966 at dilution of 1/2000.
The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.
The image shows intense enterocyte/goblet cell nuclear positivity, confined to the crypts of Lieberkühn.
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)](https://www.abcam.com/ps/products/185/ab185966/Images/ab185966-219111-anti-sox9-antibody-epr14335-78-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)Immunofluorescence analysis of 4% paraformaldehyde fixed SW480 (Human colorectal adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor®555) used as secondary antibody at 1/200 dilution. DAPI staining shown in blue.
Protocols
References
This product has been referenced in:
- Arora H et al. Subcutaneous Leydig Stem Cell Autograft: A Promising Strategy to Increase Serum Testosterone. Stem Cells Transl Med 8:58-65 (2019). Read more (PubMed: 30280521) »
- Yu L et al. BMP9 stimulates joint regeneration at digit amputation wounds in mice. Nat Commun 10:424 (2019). Read more (PubMed: 30723209) »
