Name: Anti-SOX9 antibody [EPR14335-78]
Brand: RabMAb
SKU: ab185966
Rating: 5 (26 reviews)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14335-78] to SOX9
Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

Product name

Anti-SOX9 antibody [EPR14335-78]
See all SOX9 primary antibodies
Description

Rabbit monoclonal [EPR14335-78] to SOX9
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
Species reactivity

Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Dog, Pig, Common marmoset
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- IHC-P: Mouse eye tissue. Rat, mouse and human colon tissue. Human breast carcinoma tissue. WB: SW480 and PC-3 cell lysate. ICC/IF: SW480, F9 and PC-3 cells. Flow Cyt (intra): PC-3 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14335-78
Isotype

IgG
Research areas

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Conjugation kits
- PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
- APC Conjugation Kit - Lightning-Link® (ab201807)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- SW480 whole cell lysate (ab3957)
Recombinant Protein
- Recombinant Human SOX9 protein (ab131911)
Related Products
- Prestained Protein Ladder – Broad molecular weight (10-245 kDa) (ab116028)
- Recombinant Human SOX9 protein (ab131911)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185966 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt (Intra)		Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF	(5)	Use a concentration of 5 µg/ml.
WB	(4)	1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P	(6)	1/1000. Perform heat mediated antigen retrieval with citrate buffer.

Notes
Flow Cyt (Intra) Use at an assay dependent concentration. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF Use a concentration of 5 µg/ml.
WB 1/1000 - 1/10000. Detects a band of approximately 70 kDa (predicted molecular weight: 56 kDa).
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer.

Function

Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes.
Involvement in disease

Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession P48436 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 403464 Dog
- Entrez Gene: 6662 Human
- Entrez Gene: 20682 Mouse
- Entrez Gene: 396840 Pig
- Entrez Gene: 140586 Rat
- Omim: 608160 Human
- SwissProt: Q7YRJ7 Dog
- SwissProt: P48436 Human
- SwissProt: Q04887 Mouse
- SwissProt: O18896 Pig
- Unigene: 647409 Human
- Unigene: 286407 Mouse
see all
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
- CMPD 1 antibody
- CMPD1 antibody
- SOX 9 antibody
- Sox9 antibody
- SOX9_HUMAN antibody
- SRA 1 antibody
- SRA1 antibody
- SRXX2 antibody
- SRXY10 antibody
- SRY (sex determining region Y) box 9 (campomelic dysplasia autosomal antibody
- SRY (sex determining region Y) box 9 antibody
- SRY (sex determining region Y)-box 9 antibody
- SRY (sex-determining region Y)-box 9 protein antibody
- SRY related HMG box gene 9 antibody
- Transcription factor SOX 9 antibody
- Transcription factor SOX-9 antibody
- transcription factor SOX9 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

ab185966 staining SOX9 in developing eye of mouse tissue section by Immunohistochemistry (PFA perfusion fixed frozen sections).

Tissue samples were fixed by perfusion with formaldehyde, cut into 20 micron slices, blocked with 2% BSA for 10 minutes at 21°C and antigen retrieval was by heat mediation in citrate buffer. The sample was incubated with primary antibody (1/1000 in PBS) at 21°C for 4 hours. A biotin-conjugated goat anti-rabbit polyclonal (1/300) was used as the secondary antibody.
See Abreview
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/20000 dilution + SW480 (Human colorectal adenocarcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

ab185966 staining Sox9 in F9 (Mouse embryonic testicular cancer cell line) cells.

The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab185966 at a 5 μg/ml concentration and ab7291, mouse monoclonal [DM1A] to alpha Tubulin, at 1μg/ml concentration, followed by a further incubation at room temperature for 1 h with an anti-rabbit AlexaFluor^® 488 (ab150081) at 2 μg/ml (shown in green) and an anti-mouse AlexaFluor^® 594 (ab150120) at 2 μg/ml (shown in pseudocolor red). Nuclear DNA was labeled with DAPI (shown in blue).

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Flow Cytometry (Intracellular) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Intracellular Flow Cytometry analysis of PC-3 (human prostate adenocarcinoma) cells labeling SOX9 with purified ab185966 at 1/120 (red).

Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody. Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Anti-SOX9 antibody [EPR14335-78] (ab185966) at 1/5000 dilution + PC-3 (Human prostate adenocarcinoma cell line) cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 56 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded rat colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunofluorescence analysis of 4% paraformaldehyde fixed PC-3 (Human prostate adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution (Left panel, red).

Goat anti Rabbit IgG (Alexa Fluor^®555) used as secondary antibody at 1/200 dilution. DAPI staining (Right panel, blue).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded mouse colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded human colon tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue labeling SOX9 with ab185966 at 1/1000 dilution. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX9 antibody [EPR14335-78] (ab185966)This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

Immunohistochemical analysis of Formalin/PFA-fixed paraffin-embedded pig small intestine sections labeling SOX9 with ab185966 at dilution of 1/2000.

The secondary antibody used was a polyclonal goat anti-rabbit biotin conjugated antibody at a dilution of 1/300. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using citric acid.

The image shows intense enterocyte/goblet cell nuclear positivity, confined to the crypts of Lieberkühn.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-SOX9 antibody [EPR14335-78] (ab185966)

Immunofluorescence analysis of 4% paraformaldehyde fixed SW480 (Human colorectal adenocarcinoma cell line) cells labeling SOX9 with ab185966 at 1/250 dilution. Goat anti Rabbit IgG (Alexa Fluor^®555) used as secondary antibody at 1/200 dilution. DAPI staining shown in blue.
Anti-SOX9 antibody [EPR14335-78] (ab185966)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab185966? Please let us know so that we can cite the reference in this datasheet.

ab185966 has been referenced in 107 publications.

Yang L et al. Nsun4 and Mettl3 mediated translational reprogramming of Sox9 promotes BMSC chondrogenic differentiation. Commun Biol 5:495 (2022). WB ; Rat . PubMed: 35614315
Wang Y et al. FSH directly regulates chondrocyte dedifferentiation and cartilage development. J Endocrinol 248:193-206 (2021). PubMed: 33295881
Olsen RR et al. ASCL1 represses a SOX9+ neural crest stem-like state in small cell lung cancer. Genes Dev 35:847-869 (2021). PubMed: 34016693
Zhu W et al. Silencing of the long noncoding RNA LINC01132 alleviates the oncogenicity of epithelial ovarian cancer by regulating the microRNA-431-5p/SOX9 axis. Int J Mol Med 48:N/A (2021). PubMed: 34132375
Li X et al. MicroRNA-10a-3p Improves Cartilage Degeneration by Regulating CH25H-CYP7B1-RORa Mediated Cholesterol Metabolism in Knee Osteoarthritis Rats. Front Pharmacol 12:690181 (2021). PubMed: 34149433

View all Publications for this product

Customer reviews and Q&As

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1-10 of 26 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Mouse Tissue sections (E11.5 mouse brain)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6.0

Permeabilization

Yes - 0.3% Triton X-100 in PBS

Specification

E11.5 mouse brain

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 22 2021

Application

Immunohistochemistry free floating

Sample

Rat Tissue sections (Cerebellum)

Specification

Cerebellum

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted May 04 2021

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Mouse Tissue sections (Cerebellum)

Antigen retrieval step

None

Permeabilization

Yes - PBS 1x, Triton X-100 1%

Specification

Cerebellum

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Formaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 21 2021

Application

Immunohistochemistry (Frozen sections)

Sample

Human Tissue sections (embryo 9 weeks of gestation)

Permeabilization

Yes - Triton

Specification

embryo 9 weeks of gestation

Blocking step

(agent) for 1 hour(s) and 0 minute(s) · Concentration: 0.25% · Temperature: 21°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

Submitted Jan 20 2021

Application

IHC - Wholemount

Sample

Mouse Embryo (E12.5 whole embryo)

Specification

E12.5 whole embryo

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

Application

IHC - Wholemount

Sample

Human Embryo (whole lung (age PCW7))

Specification

whole lung (age PCW7)

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Alain Chedotal

Verified customer

Submitted Nov 23 2020

Application

Immunohistochemistry (PFA perfusion fixed frozen sections)

Sample

Rat Tissue sections (Cerebellum)

Antigen retrieval step

None

Permeabilization

Yes - PBS 1x, Triton X-100 1%

Specification

Cerebellum

Blocking step

BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Fixative

Paraformaldehyde

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 04 2020

Application

Western blot

Sample

Rat Tissue lysate - whole (Cerebellum)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

Cerebellum

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Mar 15 2020

Application

Western blot

Sample

Mouse Tissue lysate - whole (Liver)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

40 µg

Specification

Liver

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 26 2020

Application

Western blot

Sample

Human Cell lysate - whole cell (HEK293)

Gel Running Conditions

Reduced Denaturing (4-15% gradient)

Loading amount

20 µg

Specification

HEK293

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Feb 17 2020

1-10 of 26 Abreviews or Q&A

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Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Conjugation kits

Isotype control

Positive Controls

Recombinant Protein

Related Products

Applications

The Abpromise guarantee

Target

Function

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

Certificate of Compliance

References (107)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry free floating abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (Frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

IHC - Wholemount abreview for Anti-SOX9 antibody [EPR14335-78]

IHC - Wholemount abreview for Anti-SOX9 antibody [EPR14335-78]

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]

Western blot abreview for Anti-SOX9 antibody [EPR14335-78]