Anti-SOX9 (phospho S181) antibody (ab59252)
Key features and details
- Rabbit polyclonal to SOX9 (phospho S181)
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-SOX9 (phospho S181) antibody
See all SOX9 primary antibodies -
Description
Rabbit polyclonal to SOX9 (phospho S181) -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human SOX9 aa 100-200 (phospho S181). (R-K-SP-V-K).
Database link: P48436 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Purification notes
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against the non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab59252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P |
1/50 - 1/100.
|
|
WB | (2) |
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).
|
Notes |
---|
IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa). |
Target
-
Function
Plays an important role in the normal skeletal development. May regulate the expression of other genes involved in chondrogenesis by acting as a transcription factor for these genes. -
Involvement in disease
Defects in SOX9 are the cause of campomelic dysplasia (CMD1) [MIM:114290]. CMD1 is a rare, often lethal, dominantly inherited, congenital osteochondrodysplasia, associated with male-to-female autosomal sex reversal in two-thirds of the affected karyotypic males. A disease of the newborn characterized by congenital bowing and angulation of long bones, unusually small scapulae, deformed pelvis and spine and a missing pair of ribs. Craniofacial defects such as cleft palate, micrognatia, flat face and hypertelorism are common. Various defects of the ear are often evident, affecting the cochlea, malleus incus, stapes and tympanum. Most patients die soon after birth due to respiratory distress which has been attributed to hypoplasia of the tracheobronchial cartilage and small thoracic cage. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 6662 Human
- Omim: 608160 Human
- SwissProt: P48436 Human
- Unigene: 647409 Human
-
Alternative names
- campomelic dysplasia autosomal sex reversal antibody
- CMD 1 antibody
- CMD1 antibody
see all
Images
-
All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/5000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HepG2 cell lysate treated with UV
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (H+L) HRP at 1/10000 dilution
Predicted band size: 56 kDaLoading control = GAPDH
10% SDS-PAGE
Primary incubation: 4°C overnight
-
ab59252 at 1/50 dilution staining SOX9 in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
-
All lanes : Anti-SOX9 (phospho S181) antibody (ab59252) at 1/500 dilution
Lane 1 : 293 cell extracts treated
with PBS (60mins)
Lane 2 : 293 cell extracts treated
with PBS (60mins) with immunizing phospho-peptide
Predicted band size: 56 kDa
Observed band size: 56 kDa
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (20)
ab59252 has been referenced in 20 publications.
- Lo WC et al. Platelet-Derived Biomaterials Inhibit Nicotine-Induced Intervertebral Disc Degeneration Through Regulating IGF-1/AKT/IRS-1 Signaling Axis. Cell Transplant 30:9636897211045319 (2021). PubMed: 34586895
- Xu Z et al. Agonism of GPR120 prevented IL-1ß-induced reduction of extracellular matrix through SOX-9. Aging (Albany NY) 12:12074-12085 (2020). PubMed: 32580167
- Gallina D & Lincoln J Dynamic Expression Profiles of Sox9 in Embryonic, Post Natal, and Adult Heart Valve Cell Populations. Anat Rec (Hoboken) 302:108-116 (2019). PubMed: 30412364
- Lam CJ et al. Highly Proliferative a-Cell-Related Islet Endocrine Cells in Human Pancreata. Diabetes 67:674-686 (2018). PubMed: 29326366
- Stelcer E et al. Expression of Pluripotency Genes in Chondrocyte-Like Cells Differentiated from Human Induced Pluripotent Stem Cells. Int J Mol Sci 19:N/A (2018). PubMed: 29439516