Anti-SRD5A2 antibody (ab27469)
Key features and details
- Goat polyclonal to SRD5A2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SRD5A2 antibody
See all SRD5A2 primary antibodies -
Description
Goat polyclonal to SRD5A2 -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
QVQCQQSPVLAG
, corresponding to N terminal amino acids 2-13 of Human SRD5A2 -
Positive control
- Human testes or prostate lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27469 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 28 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. -
Tissue specificity
Expressed in high levels in the prostate and many other androgen-sensitive tissues. -
Involvement in disease
Defects in SRD5A2 are the cause of pseudovaginal perineoscrotal hypospadias (PPSH) [MIM:264600]. A form of male pseudohermaphroditism in which 46,XY males show ambiguous genitalia at birth, including perineal hypospadias and a blind perineal pouch, and develop masculinization at puberty. The name of the disorder stems from the finding of a blind-ending perineal opening resembling a vagina and a severely hypospadiac penis with the urethra opening onto the perineum. -
Sequence similarities
Belongs to the steroid 5-alpha reductase family. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 6716 Human
- Omim: 607306 Human
- SwissProt: P31213 Human
- Unigene: 458345 Human
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Alternative names
- 3 oxo 5 alpha steroid 4 dehydrogenase 2 antibody
- 3-oxo-5-alpha-steroid 4-dehydrogenase 2 antibody
- 5 alpha SR2 antibody
see all
Images
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Anti-SRD5A2 antibody (ab27469) at 0.3 µg/ml + Human testes lysate (35ug protein in RIPA buffer)
Predicted band size: 28 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab27469 has been referenced in 5 publications.
- Samuel RM et al. Androgen Signaling Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in Men. Cell Stem Cell 27:876-889.e12 (2020). PubMed: 33232663
- Bennett GA et al. Prenatal Stress Alters Hippocampal Neuroglia and Increases Anxiety in Childhood. Dev Neurosci 37:533-45 (2015). PubMed: 26279160
- Kelleher MA et al. Changes in neuroactive steroid concentrations after preterm delivery in the Guinea pig. Reprod Sci 20:1365-75 (2013). PubMed: 23585339
- Kelleher MA et al. Sex-dependent effect of a low neurosteroid environment and intrauterine growth restriction on foetal guinea pig brain development. J Endocrinol 208:301-9 (2011). PubMed: 21149437
- Vu TT et al. Changes in human placental 5alpha-reductase isoenzyme expression with advancing gestation: effects of fetal sex and glucocorticoid exposure. Reprod Fertil Dev 21:599-607 (2009). WB ; Human . PubMed: 19383266